| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,172,268 | T→C | K1092E (AAG→GAG) | mfd ← | transcription‑repair coupling factor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,172,268 | 0 | T | C | 86.4% | 117.5 / 10.1 | 44 | K1092E (AAG→GAG) | mfd | transcription‑repair coupling factor |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base C (20/18); total (21/23) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.88e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01 | |||||||||||
ATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCCCAGTTTCT > W3110S.gb/1172212‑1172330 | aTGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCg < 1:889531/70‑1 (MQ=255)aTGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCg < 1:1204297/70‑1 (MQ=255)aTGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCg < 1:3611895/70‑1 (MQ=255)aTGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCg < 1:2529005/70‑1 (MQ=255) gctgcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCg < 1:2053378/68‑1 (MQ=255) ctgcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa < 1:1203460/71‑1 (MQ=255) ctgcGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa < 1:2566830/71‑1 (MQ=255) cGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAAt < 1:1198123/61‑1 (MQ=255) cGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAAt < 1:5828/61‑1 (MQ=255) cGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAAt < 1:4138585/61‑1 (MQ=255) cGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAAt < 1:2806077/61‑1 (MQ=255) cGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAAt < 1:2615454/61‑1 (MQ=255) cGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAAt < 1:1376318/61‑1 (MQ=255) cGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCCGCAAAt < 1:977/61‑1 (MQ=255) ggCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAAt < 1:1565212/60‑1 (MQ=255) tttttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCg < 1:389113/58‑1 (MQ=255) tttttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCg < 1:3577553/58‑1 (MQ=255) tttttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCg < 1:1276817/58‑1 (MQ=255) tttttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCg < 1:2928743/58‑1 (MQ=255) tttttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCg < 1:424210/58‑1 (MQ=255) tttttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCg < 1:2593283/58‑1 (MQ=255) tttttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCg < 1:763475/58‑1 (MQ=255) tttttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCg < 1:1093950/58‑1 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCTATCa > 1:3316452/1‑60 (MQ=39) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:753403/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:875839/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:90771/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:4127529/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:958347/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:1878558/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:132009/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:1512699/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:1520437/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:2304538/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:2141375/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:2077998/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGAt > 1:1681144/1‑58 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:3968544/1‑60 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:3387034/1‑60 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:1243671/1‑60 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:2574669/1‑60 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:1655604/1‑60 (MQ=255) tttGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTCCTCGGCAAATTCGATCa > 1:978612/1‑60 (MQ=255) gTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCCCAGTTTCt > 1:3109265/1‑71 (MQ=255) | ATGCTGCGGCTGTTTTTGCAGCAAACCAATCAACCAGGCCGGATTAACGTGATTCTTCTCGGCAAATTCGATCACCCCGCCTTTCTCATTACCTTCCAGCTTCCTGATCCCCAGTTTCT > W3110S.gb/1172212‑1172330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |