Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,173,170	C→T	R791H (CGT→CAT)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,173,170	0	C	T	100.0%	122.8 / NA	41	R791H (CGT→CAT)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/29);  total (12/29)

ATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGC  >  W3110S.gb/1173100‑1173208                                                                       |                                       ataataAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCAc                                        >  1:3154592/1‑71 (MQ=255)        aCCTGTCCTCC‑CGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:1854106/71‑1 (MQ=255)        aCCTGTCCTCC‑CGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:1849331/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:3726231/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:3749101/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:4094155/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:3053029/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:430028/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:1785226/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:1780998/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:3496209/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:820625/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:3673070/71‑1 (MQ=255)         ccTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAg                                <  1:1045100/71‑1 (MQ=255)          cTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAgg                               >  1:2599499/1‑71 (MQ=255)          cTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAgg                               >  1:2205308/1‑71 (MQ=255)          cTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAgg                               >  1:222620/1‑71 (MQ=255)             tcctccGCGCAAAATTCCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGt                              <  1:1471066/69‑1 (MQ=255)                  cgcgCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACt                       <  1:3367020/71‑1 (MQ=255)                   gcgcAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGAGAAAGGTTTTAACt                       <  1:2630241/70‑1 (MQ=255)                     gcAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGt                              <  1:1178619/61‑1 (MQ=255)                          aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCTTACTCATGGACAAAGGTTTTAACTGCCAgacg               <  1:3220609/71‑1 (MQ=255)                          aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacg               <  1:2669123/71‑1 (MQ=255)                          aTTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacg               <  1:1976242/71‑1 (MQ=255)                                 gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg        <  1:3814062/71‑1 (MQ=255)                                 gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg        <  1:3854379/71‑1 (MQ=255)                                 gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg        <  1:3082103/71‑1 (MQ=255)                                 gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg        <  1:1818835/71‑1 (MQ=255)                                 gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg        <  1:178029/71‑1 (MQ=255)                                 gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg        <  1:3589449/71‑1 (MQ=255)                                 gCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg        <  1:1610705/71‑1 (MQ=255)                                  cAGGATCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGAcgggcg        <  1:1792555/70‑1 (MQ=255)                                       tCGCCTCCCGGACCACCATGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGACGACGGGCGggcggc  <  1:2107413/71‑1 (MQ=255)                                                        aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAgacgac             >  1:191850/1‑43 (MQ=255)                                                        aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg            >  1:974635/1‑44 (MQ=255)                                                        aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg            >  1:3022964/1‑44 (MQ=255)                                                        aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg            >  1:2298651/1‑44 (MQ=255)                                                        aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg            >  1:2146298/1‑44 (MQ=255)                                                        aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg            >  1:1945452/1‑44 (MQ=255)                                                        aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg            >  1:1837704/1‑44 (MQ=255)                                                        aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg            >  1:1660462/1‑44 (MQ=255)                                                        aTGCTGTCATACTCATGGACAAAGGTTTTAACTGCCAGacgacg            >  1:1396907/1‑44 (MQ=255)                                                                       |                                       ATAATAAACCTGTCCTCCGCGCAAAATTTCACGCAGGATCGCCTCCCGGACCACCATGCTGTCATACTCACGGACAAAGGTTTTAACTGCCAGACGACGGGCGGGCGGC  >  W3110S.gb/1173100‑1173208

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑