| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,181,288 | T→G | D64E (GAT→GAG) | cobB → | deacetylase of acetyl‑CoA synthetase, NAD‑dependent |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,181,288 | 0 | T | G | 100.0% | 116.0 / NA | 33 | D64E (GAT→GAG) | cobB | deacetylase of acetyl‑CoA synthetase, NAD‑dependent |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (17/16); total (17/16) | |||||||||||
TACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGATCCTGA > W3110S.gb/1181224‑1181353 | tACTCGGACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCtg > 1:531607/1‑71 (MQ=255) cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCtgtg > 1:3995243/1‑71 (MQ=255) cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCtgtg > 1:1831762/1‑71 (MQ=255) cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCtgtg > 1:2411439/1‑71 (MQ=255) cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCtgtg > 1:3981707/1‑71 (MQ=255) cTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCtgtg > 1:3462060/1‑71 (MQ=255) acacGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAAc < 1:2733020/70‑1 (MQ=255) aCAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAAc < 1:4109267/70‑1 (MQ=255) aCAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAAc < 1:3926403/70‑1 (MQ=255) aCAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAAc < 1:3539235/70‑1 (MQ=255) ggggCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaa > 1:1882942/1‑63 (MQ=255) ggggCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCg > 1:986010/1‑71 (MQ=255) cGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGc > 1:1700796/1‑70 (MQ=255) cGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGc > 1:3662024/1‑70 (MQ=255) cGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGc > 1:3399670/1‑70 (MQ=255) cGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGc > 1:2952630/1‑70 (MQ=255) cGGAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGc > 1:2897584/1‑70 (MQ=255) gAATCAGGTATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAAc > 1:1804659/1‑71 (MQ=255) tATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGaa > 1:3686867/1‑52 (MQ=255) tATTCGTACCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGaa > 1:1726996/1‑52 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaagaa < 1:2950768/38‑1 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaagaa < 1:3518768/38‑1 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaagaa < 1:2742548/38‑1 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaagaa < 1:617453/38‑1 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaagaa < 1:254052/38‑1 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaagaa < 1:2500179/38‑1 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaagaa < 1:543822/38‑1 (MQ=255) ttCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaagaa < 1:600189/38‑1 (MQ=255) tCGTACCTTTCGCGCCGCAGAGGGCCTGTGGgaagaa < 1:2709149/37‑1 (MQ=255) aCCTTTCGCGCCGCAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGa > 1:1712382/1‑71 (MQ=255) gcAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGATCCTGa < 1:3323663/71‑1 (MQ=255) gcAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGATCCTGa < 1:3683252/71‑1 (MQ=255) cAGAGGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGATCCTGa < 1:1400637/70‑1 (MQ=255) | TACTCGTACTGACAGGGGCAGGAATTTCTGCGGAATCAGGTATTCGTACCTTTCGCGCCGCAGATGGCCTGTGGGAAGAACATCGGGTTGAAGATGTGGCAACGCCGGAAGGTTTCGATCGCGATCCTGA > W3110S.gb/1181224‑1181353 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |