Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,244,320	C→T	C113Y (TGC→TAC)	ldcA ←	L,D‑carboxypeptidase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,244,320	0	C	T	100.0%	77.6 / NA	25	C113Y (TGC→TAC)	ldcA	L,D‑carboxypeptidase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (18/7);  total (18/7)

GCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACC  >  W3110S.gb/1244254‑1244385                                                                   |                                                                  gcgcCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg                                                               <  1:116723/71‑1 (MQ=255)                        ggggCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTg                                                               <  1:103221/48‑1 (MQ=255)                         gggCCGCTAAAGGTGATGACATTGCCATGCGCCATAAGACCGTACTGAATGGCGGTAAAATCGCTATGTcc                                       >  1:1508745/1‑71 (MQ=255)                         gggCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTc                                        >  1:1174644/1‑70 (MQ=255)                               cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAAt                                 <  1:907541/71‑1 (MQ=255)                               cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAAt                                 <  1:3448643/71‑1 (MQ=255)                               cTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAAt                                 <  1:2030726/71‑1 (MQ=255)                                       gatgaCATTGCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACg                         >  1:1301645/1‑71 (MQ=255)                                                gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt                >  1:2306821/1‑71 (MQ=255)                                                gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt                >  1:1677207/1‑71 (MQ=255)                                                gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt                >  1:2526181/1‑71 (MQ=255)                                                gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgtt                >  1:352434/1‑71 (MQ=255)                                                gCCATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgt                 >  1:3606902/1‑70 (MQ=255)                                                gCCATGAGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgt                 >  1:527996/1‑70 (MQ=255)                                                  cATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgttgt              >  1:1170336/1‑71 (MQ=255)                                                  cATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgttgt              >  1:77803/1‑71 (MQ=255)                                                  cATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgttgt              >  1:508294/1‑71 (MQ=255)                                                  cATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgttgt              >  1:3335410/1‑71 (MQ=255)                                                  cATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgttgt              >  1:3185245/1‑71 (MQ=255)                                                  cATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgttgt              >  1:2934698/1‑71 (MQ=255)                                                  cATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgttgt              >  1:2400699/1‑71 (MQ=255)                                                  cATGCGCCAGAAGACCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCAtgttgt              >  1:2355661/1‑71 (MQ=255)                                                          agaagaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGc      <  1:1290081/71‑1 (MQ=255)                                                             agaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccac   >  1:687321/1‑71 (MQ=255)                                                              gaCCGTACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGccacc  <  1:3229099/71‑1 (MQ=255)                                                                   |                                                                  GCGCCAAAGTTTGCTACCAGCATGGGGCCGCTAAAGGTGATGACATTGCCATGCGCCAGAAGACCGCACTGAATGGCGGTAAAATCGCTATGTCCGCAAATGAGCAACGGGTCATGTTGTTGGCGAGCCACC  >  W3110S.gb/1244254‑1244385

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑