Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,303,633	G→T	Q246Y (CAG→TAT)	oppA →	oligopeptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,303,633	0	G	T	100.0%	178.1 / NA	56	Q246Y (CAG→TAT)	oppA	oligopeptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (28/28);  total (28/28)

TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAA  >  W3110S.gb/1303566‑1303693                                                                    |                                                             tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                           <  1:1244104/71‑1 (MQ=255) tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                           <  1:1276973/71‑1 (MQ=255) tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                           <  1:2464374/71‑1 (MQ=255) tAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTa                                                           <  1:3418283/71‑1 (MQ=255)            aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:2728338/1‑71 (MQ=255)            aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:1105107/1‑71 (MQ=255)            aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:2784798/1‑71 (MQ=255)            aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:577863/1‑71 (MQ=255)            aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:2968814/1‑71 (MQ=255)            aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:1689367/1‑71 (MQ=255)            aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:3640987/1‑71 (MQ=255)            aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:3553406/1‑71 (MQ=255)            aTCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:3610720/1‑71 (MQ=255)            aTCGTTCTTGAACGCAGACCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGcc                                                >  1:1374480/1‑71 (MQ=255)               gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                             <  1:3064984/71‑1 (MQ=255)               gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                             <  1:1953316/71‑1 (MQ=255)               gTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTAt                                             <  1:3489666/71‑1 (MQ=255)                     gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGc                                          <  1:939168/68‑1 (MQ=255)                     gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                         <  1:1458451/69‑1 (MQ=255)                     gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                         <  1:3229138/69‑1 (MQ=255)                     gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                         <  1:4000661/69‑1 (MQ=255)                     gAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                         <  1:1315078/69‑1 (MQ=255)                                gACCTACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCg                            <  1:1234461/71‑1 (MQ=255)                                    tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                               <  1:3631323/64‑1 (MQ=255)                                    tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                               <  1:1074637/64‑1 (MQ=255)                                    tACTGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTa                               <  1:2725412/64‑1 (MQ=255)                                       tGGAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCt                                         <  1:2719118/51‑1 (MQ=255)                                        ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                    <  1:21876/55‑1 (MQ=255)                                        ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                    <  1:3597075/55‑1 (MQ=255)                                        ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                    <  1:2003743/55‑1 (MQ=255)                                        ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                    <  1:3351855/55‑1 (MQ=255)                                        ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                    <  1:1354339/55‑1 (MQ=255)                                        ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGa                                    <  1:2329012/55‑1 (MQ=255)                                        ggAACAACGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCAGa                                    <  1:2603174/55‑1 (MQ=255)                                          aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                  >  1:773177/1‑55 (MQ=255)                                          aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                  >  1:484943/1‑55 (MQ=255)                                          aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                  >  1:2936230/1‑55 (MQ=255)                                          aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                  >  1:2854718/1‑55 (MQ=255)                                          aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                  >  1:3825064/1‑55 (MQ=255)                                          aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                  >  1:1414353/1‑55 (MQ=255)                                          aacaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAg                                  >  1:1568457/1‑55 (MQ=255)                                          aacaacGCGAAAACCGTTATTAACTATGTAACATATTTGc                                                 >  1:2779739/1‑40 (MQ=37)                                           acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                    >  1:520575/1‑68 (MQ=255)                                           acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                    >  1:1040141/1‑68 (MQ=255)                                           acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                    >  1:3873775/1‑68 (MQ=255)                                           acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                    >  1:1514605/1‑68 (MQ=255)                                           acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                    >  1:198833/1‑68 (MQ=255)                                           acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                    >  1:3333658/1‑68 (MQ=255)                                           acaacGCGAAAACCGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAAc                    >  1:2983543/1‑68 (MQ=255)                                                        cGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggt     >  1:129663/1‑70 (MQ=255)                                                        cGTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggt     >  1:250342/1‑70 (MQ=255)                                                         gTTATTAACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAgtggtg    >  1:3509412/1‑70 (MQ=255)                                                          ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaa  <  1:3528181/71‑1 (MQ=255)                                                          ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaa  <  1:1394703/71‑1 (MQ=255)                                                          ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaa  <  1:3285365/71‑1 (MQ=255)                                                          ttattaACTATGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGaa  <  1:23953/71‑1 (MQ=255)                                                                    |                                                             TAAACGAACGAATCGTTCTTGAACGCAGCCCGACCTACTGGAACAACGCGAAAACCGTTATTAACCAGGTAACCTATTTGCCTATTGCTTCTGAAGTTACCGATGTCAACCGCTACCGTAGTGGTGAA  >  W3110S.gb/1303566‑1303693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑