Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,357,653	G→A	P391L (CCC→CTC)	sapA ←	predicted antimicrobial peptide transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,357,653	0	G	A	100.0%	57.8 / NA	20	P391L (CCC→CTC)	sapA	predicted antimicrobial peptide transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/10);  total (10/10)

AATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTC  >  W3110S.gb/1357586‑1357705                                                                    |                                                     aaTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGt                                                   <  1:1016762/71‑1 (MQ=255) aaTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGt                                                   <  1:1288401/71‑1 (MQ=255) aaTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGt                                                   <  1:2063134/71‑1 (MQ=255) aaTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGt                                                   <  1:206808/71‑1 (MQ=255) aaTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGt                                                   <  1:2697028/71‑1 (MQ=255) aaTCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGt                                                   <  1:2860202/71‑1 (MQ=255)   tCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTc                                                 <  1:3211802/71‑1 (MQ=255)       cacTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTCCACg                                             <  1:2203828/71‑1 (MQ=255)        acTTTTACGCCAACCTGCTCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTcc                                                >  1:3716159/1‑67 (MQ=255)        acTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTCCACGc                                            >  1:1365980/1‑71 (MQ=255)        acTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTCCACGc                                            >  1:1866901/1‑71 (MQ=255)        acTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTCCACGc                                            >  1:1943876/1‑71 (MQ=255)        acTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTCCACGc                                            >  1:472419/1‑71 (MQ=255)        acTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTCCACGc                                            >  1:2257134/1‑71 (MQ=255)        acTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTCCACGc                                            >  1:3545857/1‑71 (MQ=255)                         cgcCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGGTCCACGCCTGCGAACGTGTggg                             <  1:937233/69‑1 (MQ=255)                                tCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTCCACGCCTGCGAACGTGTGGGCACCCACAg                    >  1:2210692/1‑71 (MQ=255)                                tCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGAGGTTCCACGCCTGCGAACGTGTGGGCACCCACAg                    >  1:115373/1‑71 (MQ=255)                                                  gCAGTTTTCAGTGGACTGAGGTTCCACGCCTGCGAACGTGTggg                             <  1:2718333/44‑1 (MQ=255)                                                     gTTTTCAGTGGACTGAGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTc  >  1:4106825/1‑68 (MQ=255)                                                                    |                                                     AATCACCACTTTTACGCCAACCTGCGCCATATCCGCCTGAATCAGTTCGGCAGTTTTCAGTGGACTGGGGTTCCACGCCTGCGAACGTGTGGGCACCCACAGTTTCAGCGTTAAATTTTC  >  W3110S.gb/1357586‑1357705

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑