| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,373,368 | G→A | R480H (CGT→CAT) | ycjM → | predicted glucosyltransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,373,368 | 0 | G | A | 88.0% | 64.8 / 2.4 | 25 | R480H (CGT→CAT) | ycjM | predicted glucosyltransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/3); new base A (15/7); total (15/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.22e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGT > W3110S.gb/1373303‑1373431 | aCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:916633/1‑69 (MQ=255)aCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:513406/1‑69 (MQ=255)aCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:1447929/1‑69 (MQ=255)aCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:3656848/1‑69 (MQ=255)aCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:3532193/1‑69 (MQ=255)aCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:2931960/1‑69 (MQ=255)aCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:1251720/1‑69 (MQ=255)aCTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTtcatcg > 1:2332462/1‑69 (MQ=255) gAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc > 1:284392/1‑69 (MQ=255) gAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAAc > 1:1597260/1‑69 (MQ=255) aGCTACATTAAGTCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAg < 1:3398267/70‑1 (MQ=255) aGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAg < 1:282636/70‑1 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGAtaataa > 1:3900540/1‑70 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:1371557/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:2183242/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:2891330/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:3637745/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:3535270/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:1610367/1‑71 (MQ=255) cGGTATATCATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:3530386/1‑71 (MQ=255) cGGTATATAATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:4013785/1‑71 (MQ=255) cGGTATAACATGAGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATaataat > 1:169738/1‑71 (MQ=255) aGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGAt < 1:3499346/71‑1 (MQ=255) aGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGAt < 1:3358952/71‑1 (MQ=255) aGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGAt < 1:1645221/71‑1 (MQ=255) aGTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGAt < 1:2531887/71‑1 (MQ=255) gTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGAt < 1:2081213/70‑1 (MQ=255) gTTGTCGCGTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGAt < 1:1422320/70‑1 (MQ=255) gTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTATCATTGATACGATTAAt > 1:3908580/1‑71 (MQ=255) gTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAt > 1:1909216/1‑71 (MQ=255) gTTTAATTACACTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAAt > 1:1614672/1‑71 (MQ=255) cacTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:897042/70‑1 (MQ=255) cacTTCATCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGt < 1:1926976/70‑1 (MQ=255) | ACTGAACGATGAAGCTACATTAAGGCATGCGGTATATCATGAGTTGTCGCGTTTAATTACACTTCGTCGCAGCCATAACGAGTTTCATCCGGATAATAATTTTACCATTGATACGATTAATTCATCCGT > W3110S.gb/1373303‑1373431 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |