Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,376,428	G→A	V209M (GTG→ATG)	ycjP →	predicted sugar transporter subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,376,428	0	G	A	96.7%	90.0 / ‑3.8	30	V209M (GTG→ATG)	ycjP	predicted sugar transporter subunit
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (12/17);  total (13/17)
Fisher's exact test for biased strand distribution p-value = 4.33e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01

ACCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCTTA  >  W3110S.gb/1376374‑1376490                                                       |                                                               aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTa                                                >  1:1644712/1‑71 (MQ=255) aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTa                                                >  1:563871/1‑71 (MQ=255) aCCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTa                                                >  1:4000076/1‑71 (MQ=255)   cGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATc                                              <  1:3720169/71‑1 (MQ=255)   cGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATc                                              <  1:1371236/71‑1 (MQ=255)   cGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATc                                              <  1:1488033/71‑1 (MQ=255)   cGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATc                                              <  1:1833272/71‑1 (MQ=255)   cGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATc                                              <  1:3029349/71‑1 (MQ=255)     tGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTg                                            <  1:3577080/71‑1 (MQ=255)     tGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTg                                            <  1:189493/71‑1 (MQ=255)     tGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTg                                            <  1:3566977/71‑1 (MQ=255)                            atatCCGTGTTCGTTTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAg                     >  1:2767521/1‑71 (MQ=255)                            atatCCGTGTTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAg                     >  1:3219208/1‑71 (MQ=255)                                   tgtTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAg               >  1:734319/1‑70 (MQ=255)                                   tgtTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAg               >  1:3625423/1‑70 (MQ=255)                                   tgtTCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAg               >  1:1063197/1‑70 (MQ=255)                                     ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAg                     <  1:3508888/62‑1 (MQ=255)                                     ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCaa            <  1:3259585/71‑1 (MQ=255)                                     ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCaa            <  1:3328981/71‑1 (MQ=255)                                     ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCaa            <  1:3485144/71‑1 (MQ=255)                                     ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCaa            <  1:2527715/71‑1 (MQ=255)                                     ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCaa            <  1:3880363/71‑1 (MQ=255)                                     ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCaa            <  1:1242942/71‑1 (MQ=255)                                     ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCaa            <  1:4125129/71‑1 (MQ=255)                                     ttCGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCaa            <  1:1044448/71‑1 (MQ=255)                                       cGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGa                                  >  1:3834529/1‑47 (MQ=255)                                       cGTCTACTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGAt                                 >  1:3210614/1‑48 (MQ=255)                                             cTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCt    >  1:1845062/1‑71 (MQ=255)                                             cTGCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCt    >  1:126165/1‑71 (MQ=255)                                               gCTTTATGATGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCTTa  >  1:1707325/1‑71 (MQ=255)                                                       |                                                               ACCGTGCCGTTGGCGATGTCCGGGCTGATATCCGTGTTCGTCTACTGCTTTATGGTGGCGTGGAACGACTATCTGTTTGCGTCGATTTTCCTCTCCAGCGCCAGCAATTTCACCTTA  >  W3110S.gb/1376374‑1376490

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑