Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,401,010	T→C	K77K (AAA→AAG)	fnr ←	DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,401,010	0	T	C	100.0%	199.5 / NA	56	K77K (AAA→AAG)	fnr	DNA‑binding transcriptional dual regulator, global regulator of anaerobic growth
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (22/34);  total (22/34)

CTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCG  >  W3110S.gb/1400955‑1401071                                                        |                                                              cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                >  1:2038622/1‑71 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:62681/71‑1 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                >  1:2820477/1‑71 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                >  1:1396278/1‑71 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                >  1:4066075/1‑71 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:4004111/71‑1 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:1907888/71‑1 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:3872318/71‑1 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:2557102/71‑1 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:1719780/71‑1 (MQ=255) cTGCTAAATGGAAACCAGTGATTTGCTCGTAGCCTTGATCAGTGATGGTATAACTCTTAATCGTACCGGa                                                 >  1:1645575/1‑70 (MQ=255)  tGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:48077/70‑1 (MQ=255)    cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:2364184/70‑1 (MQ=255)    cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:2229437/70‑1 (MQ=255)    cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:547967/70‑1 (MQ=255)    cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:1310372/70‑1 (MQ=255)    cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:1956560/70‑1 (MQ=255)    cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCg                                              <  1:3295277/70‑1 (MQ=255)    cTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGCATAACTCTTAATCGTACCGGAGCg                                              <  1:4119992/70‑1 (MQ=255)      aaaTGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTa                                                      >  1:3732492/1‑60 (MQ=255)      aaaTGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTa                                                      >  1:3817226/1‑60 (MQ=255)         tGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATgg                                         >  1:1910567/1‑70 (MQ=255)         tGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATgg                                         >  1:2303364/1‑70 (MQ=255)          ggAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:3400038/62‑1 (MQ=255)             aaCCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:2341316/59‑1 (MQ=255)                cAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAg                                                <  1:2111823/56‑1 (MQ=255)                  gTGATTTGCTCGTCGCCTTTCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1895196/71‑1 (MQ=255)                  gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1768321/71‑1 (MQ=255)                  gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:2203478/71‑1 (MQ=255)                  gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:3523372/71‑1 (MQ=255)                  gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1562309/71‑1 (MQ=255)                  gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:2957605/71‑1 (MQ=255)                  gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1507716/71‑1 (MQ=255)                  gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:3289612/71‑1 (MQ=255)                  gTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGa                               <  1:1508081/71‑1 (MQ=255)                     aTTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGct                                <  1:2023461/67‑2 (MQ=255)                         gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:317274/70‑1 (MQ=255)                         gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:3509453/70‑1 (MQ=255)                         gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:2578989/70‑1 (MQ=255)                         gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:2149860/70‑1 (MQ=255)                         gCTCGTCGCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAg                         <  1:2291690/70‑1 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:1011512/1‑71 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:1505134/1‑71 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:1701049/1‑71 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:3690148/1‑71 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:3636099/1‑71 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:3513515/1‑71 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:2006504/1‑71 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:3289568/1‑71 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCAc                 >  1:2798857/1‑71 (MQ=255)                                gCCTTGCTCAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGAATAAAGCGATTTAAGTTCATCAc                 >  1:1167211/1‑71 (MQ=255)                                        cAGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGGGa                               <  1:1113947/49‑1 (MQ=255)                                         aGTGATGGTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTa         <  1:463092/70‑1 (MQ=255)                                               ggTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCg  >  1:3944863/1‑71 (MQ=255)                                               ggTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCg  >  1:2994289/1‑71 (MQ=255)                                               ggTATAACTCTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCg  >  1:940281/1‑71 (MQ=255)                                                        |                                                              CTGCTAAATGGAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCG  >  W3110S.gb/1400955‑1401071

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑