| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,463,502 | C→T | P299L (CCG→CTG) | paaJ → | predicted beta‑ketoadipyl CoA thiolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,463,502 | 0 | C | T | 95.0% | 53.3 / ‑4.2 | 20 | P299L (CCG→CTG) | paaJ | predicted beta‑ketoadipyl CoA thiolase |
| Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (5/14); total (5/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CGGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATTGA > W3110S.gb/1463441‑1463568 | cgGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGc < 1:815751/71‑1 (MQ=255) ccATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGt < 1:129017/67‑1 (MQ=255) ccATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGt < 1:1730256/67‑1 (MQ=255) ccATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGt < 1:34770/67‑1 (MQ=255) cgccgGGGTGGAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGt < 1:864094/57‑1 (MQ=255) gAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTAt > 1:1248012/1‑71 (MQ=255) gAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTAt > 1:3262260/1‑71 (MQ=255) gAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTAt > 1:2549477/1‑71 (MQ=255) gAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTAt > 1:2192191/1‑71 (MQ=255) gAACCGCGCCTGATGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTAt > 1:1340714/1‑71 (MQ=255) ttaTGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAAcgcg < 1:2910559/46‑1 (MQ=39) tGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGAc < 1:2838807/71‑1 (MQ=255) tGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGAc < 1:2077626/71‑1 (MQ=255) tGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGAc < 1:3930032/71‑1 (MQ=255) tGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGAc < 1:488518/71‑1 (MQ=255) tGGGGCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGAc < 1:1120887/71‑1 (MQ=255) ggggCTTGGTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGAc < 1:640488/70‑1 (MQ=255) ggTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGTACGTGATtg < 1:3148688/70‑1 (MQ=255) gTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATtga < 1:3492950/70‑1 (MQ=255) gTCTGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATtga < 1:3612779/70‑1 (MQ=255) | CGGGCGCGTATCGTAGCCATGGCAACCGCCGGGGTGGAACCGCGCCTGATGGGGCTTGGTCCGGTGCCTGCAACTCGCCGGGTGCTGGAACGCGCAGGGCTGAGTATTCACGATATGGACGTGATTGA > W3110S.gb/1463441‑1463568 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |