Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,477,935	G→A	E360K (GAA→AAA)	ydbD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,477,935	0	G	A	100.0%	78.8 / NA	26	E360K (GAA→AAA)	ydbD	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/15);  total (11/15)

GAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTGAAAATCTCCCTCTGGATATCGTCATGACTCTGGCGAAAAGAGATTGGGATATGATCA  >  W3110S.gb/1477872‑1477992                                                                |                                                          gAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAAtct                                                    >  1:921404/1‑71 (MQ=255) gAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAAtct                                                    >  1:2932808/1‑71 (MQ=255) gAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAAtct                                                    >  1:3158279/1‑71 (MQ=255) gAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAAtct                                                    >  1:2095584/1‑71 (MQ=255) gAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAAtct                                                    >  1:1655423/1‑71 (MQ=255) gAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAAtc                                                     >  1:593960/1‑70 (MQ=255) gAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAAtc                                                     >  1:2438556/1‑70 (MQ=255) gAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAAtc                                                     >  1:3255007/1‑70 (MQ=255) gAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAAtc                                                     >  1:2932629/1‑70 (MQ=255)   aataatGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAATCTcc                                                  <  1:2839428/71‑1 (MQ=255)   aataatGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAATCTcc                                                  <  1:2877169/71‑1 (MQ=255)                    gtcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATg                                 <  1:1900440/71‑1 (MQ=255)                    gtcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATg                                 <  1:2120618/71‑1 (MQ=255)                    gtcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATg                                 <  1:4002474/71‑1 (MQ=255)                    gtcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATg                                 <  1:2350213/71‑1 (MQ=255)                    gtcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATg                                 <  1:2477805/71‑1 (MQ=255)                    gtcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATg                                 <  1:3709604/71‑1 (MQ=255)                    gtcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATg                                 <  1:1040355/71‑1 (MQ=255)                     tcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTaaaa                                                        <  1:2856123/47‑1 (MQ=255)                     tcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTaaaa                                                        <  1:2541551/47‑1 (MQ=255)                     tcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTaaaa                                                        <  1:2294231/47‑1 (MQ=255)                     tcgtcTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTaaaa                                                        <  1:1844092/47‑1 (MQ=255)                                            cGTTGAATCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATg                                 <  1:3925645/47‑1 (MQ=255)                                            cGTTGAATCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATGACTCTGGCGAAAAGAGATTGGGat         <  1:2599748/71‑1 (MQ=255)                                                   tCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATGACTCTGGCGAAAAGAGATTGGGATATGATCa  >  1:3394931/1‑71 (MQ=255)                                                   tCTTGCGCTACGTAAAAATCTCCCTCTGGATATCGTCATGACTCTGGCGAAAAGAGATTGGGATATGATCa  >  1:252398/1‑71 (MQ=255)                                                       gcgcTACGTAAAAATCTCCCTCTGGATATCGTCATGACTCTGGCGAAAAGAGATTGGGATATGATCa  <  1:3720783/67‑1 (MQ=255)                                                       gcgcTACGTAAAAATCTCCCTCTGGATATCGTCATGACTCTGGCGAAAAGAGATTGGGATATGATCa  <  1:457734/67‑1 (MQ=255)                                                       gcgcTACGTAAAAATCTCCCTCTGGATATCGTCATGACTCTGGCGAAAAGAGATTGGGATATGATCa  <  1:1174849/67‑1 (MQ=255)                                                        cgcTACGTAAAAATCTCCCTCTGGATATCGTCATGACTCTGGCGAAAAGAGATTGGGATATGATCa  <  1:3173795/66‑1 (MQ=255)                                                                |                                                          GAAATAATGACGCGAATGAGTCGTCTACCTGATGGTGTCGGTCCGTTGAATCTTGCGCTACGTGAAAATCTCCCTCTGGATATCGTCATGACTCTGGCGAAAAGAGATTGGGATATGATCA  >  W3110S.gb/1477872‑1477992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑