Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,521,063	C→T	S108F (TCC→TTC)	yncB →	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,521,063	0	C	T	100.0%	36.6 / NA	13	S108F (TCC→TTC)	yncB	predicted oxidoreductase, Zn‑dependent and NAD(P)‑binding
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (8/5);  total (8/5)

ATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGC  >  W3110S.gb/1521006‑1521128                                                          |                                                                  aTTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgatg                                                        <  1:2971129/69‑1 (MQ=255) aTTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGtgatg                                                        <  1:934978/69‑1 (MQ=255)             gCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACtt                                          >  1:2274354/1‑71 (MQ=255)             gCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACtt                                          >  1:2612280/1‑71 (MQ=255)             gCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACtt                                          >  1:3582888/1‑71 (MQ=255)             gCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACtt                                          >  1:565394/1‑71 (MQ=255)                                     gATGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCg                  <  1:1346320/71‑1 (MQ=255)                                       tGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTg                >  1:1676288/1‑71 (MQ=255)                                       tGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTg                >  1:3337074/1‑71 (MQ=255)                                       tGGCAAGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTg                >  1:3662149/1‑71 (MQ=255)                                       tGGCAAGACTATGACATATTCAGTGGTGATGATCTAGTGAAACTTGGCGATCATCCGCAAAATCCATCGTg                >  1:2213486/1‑71 (MQ=255)                                           aaGACTATGACATATTCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCg            <  1:2422905/71‑1 (MQ=255)                                                     cATATTCAGTGGTGATGATCTGGTTAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGc  <  1:1074880/71‑1 (MQ=255)                                                          |                                                                  ATTATCAGTCTGGCGACTGGGTGCTGGGCTACAGTGGATGGCAAGACTATGACATATCCAGTGGTGATGATCTGGTGAAACTTGGCGATCATCCGCAAAATCCATCGTGGTCGCTGGGTGTGC  >  W3110S.gb/1521006‑1521128

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑