Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,569,965	T→C	Q191Q (CAA→CAG)	yddW ←	predicted liprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,569,965	0	T	C	100.0%	326.5 / NA	88	Q191Q (CAA→CAG)	yddW	predicted liprotein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (58/30);  total (58/30)

AACCTCAGGGATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGTACCGG  >  W3110S.gb/1569902‑1570026                                                                |                                                              aaCCTCAGGGATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGcaca                                                         <  1:3343019/70‑1 (MQ=255)         ggATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa                                                       <  1:3364657/64‑1 (MQ=255)         ggATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa                                                       <  1:96744/64‑1 (MQ=255)         ggATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa                                                       <  1:2520217/64‑1 (MQ=255)         ggATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa                                                       <  1:392351/64‑1 (MQ=255)         ggATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa                                                       <  1:2341624/64‑1 (MQ=255)         ggATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa                                                       <  1:2325896/64‑1 (MQ=255)         ggATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATa                                                       <  1:3223187/64‑1 (MQ=255)           aTGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGa                                                     >  1:867286/1‑64 (MQ=255)           aTGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGa                                                     >  1:432231/1‑64 (MQ=255)           aTGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGa                                                     >  1:2270231/1‑64 (MQ=255)           aTGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGa                                                     >  1:2982095/1‑64 (MQ=255)           aTGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGa                                                     >  1:611172/1‑64 (MQ=255)           aTGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGAAAATAGAcag                                                  >  1:3711041/1‑65 (MQ=255)            tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGcc                                             >  1:2124014/1‑71 (MQ=255)            tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGcc                                             >  1:3077419/1‑71 (MQ=255)            tGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGcc                                             >  1:3206041/1‑71 (MQ=255)            tGCCCGGGTCGAGGACAAAGCGATCGCCAGACCTTCTGATCCAGTCGCGGTGCTGCACATAGACga                                                  >  1:3713786/1‑65 (MQ=255)              cccGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCgg                                           >  1:2090487/1‑71 (MQ=255)                           cAAAGCTATCGCCAGACGTTCTGATCCAGTCCCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGAc                                 >  1:4122950/1‑68 (MQ=255)                           cAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGAc                                 >  1:644458/1‑68 (MQ=255)                           cAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGAc                                 >  1:3773339/1‑68 (MQ=255)                           cAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCAGCACATAGACGCTCGCCGGTTGTTGAGAc                                 >  1:813120/1‑68 (MQ=255)                              aGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTg                           <  1:3263935/71‑1 (MQ=255)                              aGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTg                           <  1:2778115/71‑1 (MQ=255)                              aGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTg                           <  1:2624290/71‑1 (MQ=255)                              aGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTg                           <  1:3955289/71‑1 (MQ=255)                                 gATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        <  1:854933/71‑1 (MQ=255)                                 gATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        <  1:2848147/71‑1 (MQ=255)                                 gATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        <  1:2975040/71‑1 (MQ=255)                                 gATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        <  1:1679866/71‑1 (MQ=255)                                 gATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        <  1:2249227/71‑1 (MQ=255)                                 gATCGCCAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        <  1:20781/71‑1 (MQ=255)                                       cAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGt                  >  1:4131176/1‑71 (MQ=255)                                       cAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGt                  >  1:143314/1‑71 (MQ=255)                                       cAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGt                  >  1:927588/1‑71 (MQ=255)                                       cAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGt                  >  1:3592301/1‑71 (MQ=255)                                       cAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGt                  >  1:2942161/1‑71 (MQ=255)                                       cAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGt                  >  1:3386060/1‑71 (MQ=255)                                       cAGACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGt                  >  1:396955/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGc                                                  >  1:3784100/1‑37 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCTGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:3271712/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:4016243/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:2301589/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:226056/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:490674/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:1909025/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:615920/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:1809529/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:1449237/1‑71 (MQ=255)                                         gACGTTCTGATCCAGTCGCGGTGCTGCACATAGACGATCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTc                >  1:3034696/1‑71 (MQ=255)                                            gTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCt             <  1:1023561/71‑1 (MQ=255)                                            gTTCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCt             <  1:2457840/71‑1 (MQ=255)                                             ttCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        >  1:290315/1‑59 (MQ=255)                                             ttCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        >  1:362387/1‑59 (MQ=255)                                             ttCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        >  1:621953/1‑59 (MQ=255)                                             ttCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        >  1:3773248/1‑59 (MQ=255)                                             ttCTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCAGGTTGTTGAGACAGAGTGCTa                        >  1:2609250/1‑59 (MQ=255)                                             ttCTGATCCAGTCGCGGTGCT‑CACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           <  1:4020762/71‑1 (MQ=255)                                               cTGATCCAGTCGCGGTGCTTCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTc                     >  1:2274756/1‑60 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           >  1:903410/1‑70 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           >  1:10323/1‑70 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           <  1:3854353/70‑1 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           >  1:3800967/1‑70 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           >  1:1073559/1‑70 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           >  1:1113833/1‑70 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           <  1:2885497/70‑1 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           <  1:1761709/70‑1 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           >  1:1834269/1‑70 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           >  1:1937283/1‑70 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           >  1:234380/1‑70 (MQ=255)                                               cTGATCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           >  1:2042253/1‑70 (MQ=255)                                               cTGATCCAGTCGCGGTGCCGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGa           <  1:3625076/70‑1 (MQ=255)                                                  aTCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAg        >  1:3891302/1‑70 (MQ=255)                                                  aTCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAg        >  1:3584361/1‑70 (MQ=255)                                                  aTCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGt       <  1:2283430/71‑1 (MQ=255)                                                  aTCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGt       <  1:427572/71‑1 (MQ=255)                                                  aTCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGt       <  1:4051715/71‑1 (MQ=255)                                                  aTCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGt       <  1:1723228/71‑1 (MQ=255)                                                  aTCCAGTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGt       <  1:2915688/71‑1 (MQ=255)                                                       gTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGTACCgg  >  1:2574597/1‑71 (MQ=255)                                                       gTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGTACCgg  >  1:759550/1‑71 (MQ=255)                                                       gTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGTACCgg  >  1:1415305/1‑71 (MQ=255)                                                       gTCGCGGTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGTACCgg  >  1:1069431/1‑71 (MQ=255)                                                            ggTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        >  1:2473064/1‑44 (MQ=255)                                                            ggTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        >  1:114821/1‑44 (MQ=255)                                                            ggTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        >  1:1138374/1‑44 (MQ=255)                                                            ggTGCTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTa                        >  1:988209/1‑44 (MQ=255)                                                                |                                                              AACCTCAGGGATGCCCGGGTCGAGGACAAAGCGATCGCCAGACGTTCTGATCCAGTCGCGGTGTTGCACATAGACGCTCGCCGGTTGTTGAGACAGAGTGCTATTCAGTTCCCTGATAGTACCGG  >  W3110S.gb/1569902‑1570026

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑