| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,620,710 | C→T | intergenic (‑88/‑124) | marC ← / → marR | predicted transporter/DNA‑binding transcriptional repressor |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,620,710 | 0 | C | T | 96.5% | 260.8 / 0.4 | 85 | intergenic (‑88/‑124) | marC/marR | predicted transporter/DNA‑binding transcriptional repressor |
| Reads supporting (aligned to +/- strand): ref base C (0/3); new base T (54/28); total (54/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.55e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.59e-01 | |||||||||||
TTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACTTAT > W3110S.gb/1620650‑1620779 | ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGGTAGCCTTGTATCGCATTg > 1:3210383/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:205040/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:2293765/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:3583453/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:1871785/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:3906495/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:3497726/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:2535091/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:53802/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:1741723/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:1687870/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:2546572/1‑70 (MQ=255)ttATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:1735841/1‑70 (MQ=255)ttATCAGAGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTg > 1:2906450/1‑70 (MQ=255) taAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCg < 1:213090/70‑1 (MQ=255) taAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCg < 1:3937018/70‑1 (MQ=255) taAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCg < 1:3115082/70‑1 (MQ=255) taAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCg < 1:879749/70‑1 (MQ=255) taAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:1672170/71‑1 (MQ=255) taAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:148295/71‑1 (MQ=255) taAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:3313122/71‑1 (MQ=255) taAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:3284737/71‑1 (MQ=255) taAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:1142723/71‑1 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTg > 1:3704250/1‑60 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGATCCGATTTa > 1:1314828/1‑70 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAg > 1:2712242/1‑71 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAg > 1:219432/1‑71 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAg > 1:3143070/1‑71 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAg > 1:1920074/1‑71 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAg > 1:3789864/1‑71 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAg > 1:1796425/1‑71 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAg > 1:3735566/1‑71 (MQ=255) ggTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAg > 1:596779/1‑71 (MQ=255) aaCAAGTATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCaa > 1:1823443/1‑70 (MQ=255) aaCAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTAt > 1:824885/1‑38 (MQ=255) aaCAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCaa > 1:3655585/1‑70 (MQ=255) aaCAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCaa > 1:868902/1‑70 (MQ=255) aaCAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCaa > 1:1459473/1‑70 (MQ=255) gtCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCaaa > 1:1899057/1‑57 (MQ=255) gtCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGt > 1:1710468/1‑70 (MQ=255) gtCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGt > 1:2567365/1‑70 (MQ=255) gtCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGt > 1:3405585/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:578799/47‑1 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:849654/47‑1 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:1006235/47‑1 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:10304/47‑1 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:1590033/47‑1 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:3061012/47‑1 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:2362255/47‑1 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGa < 1:3131232/47‑1 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAAGGTGGCATCGGTc > 1:3741630/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGt > 1:3436249/1‑69 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc > 1:3723425/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc > 1:965022/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc > 1:1005954/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc > 1:3909393/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc > 1:1834440/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc > 1:1937912/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc > 1:2182031/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc > 1:2339089/1‑70 (MQ=255) tCACTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTc > 1:3271953/1‑70 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCaa < 1:3173691/70‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCaa < 1:3085988/70‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCaa < 1:1152624/70‑1 (MQ=255) aCTCTTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCaa < 1:187438/70‑1 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:680014/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:1444220/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:553243/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:3992898/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:1758855/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:338173/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:3172132/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattca > 1:2215117/1‑71 (MQ=255) ctTTAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattc > 1:1477272/1‑70 (MQ=255) cattAGCTAGCCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAattc > 1:2325066/3‑70 (MQ=255) gctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAAttcattca < 1:3206287/70‑1 (MQ=255) gctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAAttcattca < 1:1384024/70‑1 (MQ=255) gctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAAttcattca < 1:764897/70‑1 (MQ=255) gctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAAttcattca < 1:3624296/70‑1 (MQ=255) gctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAAttcattca < 1:3210031/70‑1 (MQ=255) gctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAAttcattca < 1:3516099/70‑1 (MQ=255) gctagcCTTGTATCGCATTGAACAAAACTTGAACCGATTTAACAAAACGTGGCATCGGTCAAttcattca < 1:890541/70‑1 (MQ=255) gcCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTg < 1:3032094/70‑1 (MQ=255) gcCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTg < 1:368327/70‑1 (MQ=255) gcCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTg < 1:3672920/70‑1 (MQ=255) cATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACTtat < 1:3964389/70‑1 (MQ=255) | TTATCAGCGAAAAGTATAAGGGGTAAACAAGGATAAAGTGTCACTCTTTAGCTAGCCTTGCATCGCATTGAACAAAACTTGAACCGATTTAGCAAAACGTGGCATCGGTCAATTCATTCATTTGACTTAT > W3110S.gb/1620650‑1620779 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |