Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,692,839	G→A	G425D (GGT→GAT)	ydgA →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,692,839	0	G	A	100.0%	33.0 / NA	12	G425D (GGT→GAT)	ydgA	conserved hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/6);  total (6/6)

CCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCA  >  W3110S.gb/1692790‑1692905                                                  |                                                                   ccGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCg                                                 >  1:1250794/1‑69 (MQ=255) ccGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGa                                                >  1:1648275/1‑70 (MQ=255)   ggTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGaaaa                                             <  1:3653122/71‑1 (MQ=255)    gTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGaaaa                                             <  1:3612711/70‑1 (MQ=255)             gCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGa                                    <  1:3977540/70‑1 (MQ=255)              cAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGaaa                                  <  1:4113391/71‑1 (MQ=255)                        tATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGaa                                                          >  1:1703041/1‑37 (MQ=255)                        tATGACTCAGGTAGCGAAGCTGGAAGATTATCAGGaa                                                          >  1:2955810/1‑37 (MQ=255)                                   tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGaaaaaa                                           >  1:3467899/1‑41 (MQ=255)                                   tAGCGAAGCTGGAAGATTATCAGGAAGATCAAGCGaaaaa                                            >  1:2428901/1‑40 (MQ=255)                                     gCGAAGCTGGAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGc           <  1:3017661/71‑1 (MQ=255)                                              gAAGATTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCa  <  1:403746/71‑1 (MQ=255)                                                  |                                                                   CCGGTGGATATGGCAACTGAGTTTATGACTCAGGTAGCGAAGCTGGAAGGTTATCAGGAAGATCAAGCGAAAAAACTGGCGAAACAGCAAGTTGAAGGTGCATCAGCAATGGGGCA  >  W3110S.gb/1692790‑1692905

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑