BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,753,336	T→C	T35A (ACG→GCG)	ydhW ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,753,336	0	T	C	100.0%	116.6 / NA	33	T35A (ACG→GCG)	ydhW	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (14/19); total (14/19)

TGCCCAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGTATCAACGGGGTGACGCATCCCCTGCAACCATTGCCGCTTCGCCTCGTCAACTTC  >  W3110S.gb/1753269‑1753390

tGCCCAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCAt                                                      >  1:4073613/1‑70 (MQ=255)

tGCCCAGACGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCAt                                                      >  1:1926564/1‑70 (MQ=255)

   ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc                                                  <  1:131648/71‑1 (MQ=255)

   ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc                                                  <  1:1330994/71‑1 (MQ=255)

   ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc                                                  <  1:3825556/71‑1 (MQ=255)

   ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc                                                  <  1:3805940/71‑1 (MQ=255)

   ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc                                                  <  1:3099410/71‑1 (MQ=255)

   ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc                                                  <  1:2131825/71‑1 (MQ=255)

   ccAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAAc                                                  <  1:1856051/71‑1 (MQ=255)

        cGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGt                                             >  1:1591982/1‑71 (MQ=255)

              aTGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCa                                       <  1:760000/71‑1 (MQ=255)

                       aaTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa                              <  1:754761/71‑1 (MQ=255)

                       aaTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa                              <  1:2015760/71‑1 (MQ=255)

                       aaTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa                              <  1:1496148/71‑1 (MQ=255)

                       aaTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa                              <  1:3542458/71‑1 (MQ=255)

                        aTGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCa                              <  1:1370353/70‑1 (MQ=255)

                           aaCTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGc                                        <  1:1078064/57‑1 (MQ=255)

                           aaCTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGc                                        <  1:677836/57‑1 (MQ=255)

                           aaCTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGc                                        <  1:226754/57‑1 (MQ=255)

                           aaCTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGc                                        <  1:3428298/57‑1 (MQ=255)

                              tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg                       >  1:1876011/1‑71 (MQ=255)

                              tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg                       >  1:425746/1‑71 (MQ=255)

                              tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg                       >  1:1531278/1‑71 (MQ=255)

                              tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg                       >  1:3589532/1‑71 (MQ=255)

                              tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg                       >  1:3096324/1‑71 (MQ=255)

                              tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg                       >  1:1790943/1‑71 (MQ=255)

                              tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg                       >  1:2123341/1‑71 (MQ=255)

                              tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg                       >  1:1869716/1‑71 (MQ=255)

                              tCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTg                       >  1:1874550/1‑71 (MQ=255)

                                         ttCAGCAGGTTCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAAc                            <  1:2544435/55‑1 (MQ=255)

                                                 gTTCTGGTTCGGTTTCCGCATCAACGGGGTGACGCa                                       <  1:1734504/36‑1 (MQ=37)

                                                   tCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTGCCGCTTCGCCTCGTCAACTTc  >  1:3395894/1‑71 (MQ=255)

                                                   tCTGGTTCGGTTACCGCATCAACGGGGTGACGCATCCCCTGCAACCATTGCCGCTTCGCCTCGTCAACTTc  >  1:2085092/1‑71 (MQ=255)

TGCCCAGCCGCAGAATGCTGGCGAATGAACTCAGCCAGGATTTCAGCAGGTTCTGGTTCGGTTACCGTATCAACGGGGTGACGCATCCCCTGCAACCATTGCCGCTTCGCCTCGTCAACTTC  >  W3110S.gb/1753269‑1753390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`