Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,775,080	T→C	F285F (TTT→TTC)	ydiN →	predicted transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,775,080	0	T	C	83.0%	113.3 / 15.9	47	F285F (TTT→TTC)	ydiN	predicted transporter
Reads supporting (aligned to +/- strand):  ref base T (5/3);  new base C (22/17);  total (27/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.95e-01

GGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAT  >  W3110S.gb/1775014‑1775146                                                                   |                                                                   ggTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAAc                                                                 <  1:1943822/70‑1 (MQ=255)  gTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAActc                                                               <  1:3665931/71‑1 (MQ=255)  gTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAActc                                                               <  1:1876307/71‑1 (MQ=255)   tCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAActc                                                               <  1:513422/70‑1 (MQ=255)    cTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTg                                                             >  1:625865/1‑71 (MQ=255)    cTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTg                                                             >  1:3451953/1‑71 (MQ=255)           tttATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAAct                                                                <  1:801888/61‑1 (MQ=255)           tttATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAAct                                                                <  1:1556211/61‑1 (MQ=255)           tttATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAAct                                                                <  1:3798748/61‑1 (MQ=255)           tttATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAAct                                                                <  1:2263384/61‑1 (MQ=255)             tATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAActc                                                               >  1:2489956/1‑60 (MQ=255)                  ttGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAAt                                                <  1:991668/70‑1 (MQ=255)                  ttGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAAt                                                <  1:1567851/70‑1 (MQ=255)                            actGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCa                                     >  1:2739582/1‑71 (MQ=255)                             ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc                                      <  1:3317862/69‑1 (MQ=255)                             ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc                                      <  1:1543256/69‑1 (MQ=255)                             ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc                                      <  1:3422134/69‑1 (MQ=255)                             ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc                                      <  1:2890052/69‑1 (MQ=255)                             ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc                                      <  1:1781194/69‑1 (MQ=255)                             ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAt                                    >  1:3342754/1‑71 (MQ=255)                             ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAt                                    >  1:3403821/1‑71 (MQ=255)                             ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAt                                    >  1:377374/1‑71 (MQ=255)                             ctGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCAt                                    >  1:2831349/1‑71 (MQ=255)                              tGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAAt                                                <  1:1027508/58‑1 (MQ=255)                              tGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAAt                                                <  1:2522605/58‑1 (MQ=255)                              tGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGCAACAATAACAGCAGcc                                      <  1:2792483/68‑1 (MQ=255)                               gAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGcaa                                                    >  1:272628/1‑53 (MQ=255)                               gAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGcaa                                                    >  1:1656232/1‑53 (MQ=255)                               gAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGcaa                                                    >  1:2511041/1‑53 (MQ=255)                               gAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGcaa                                                    >  1:2227435/1‑53 (MQ=255)                               gAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTCAACTCTGCACTGGcaa                                                    >  1:2055966/1‑53 (MQ=255)                                                 cATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTtctc                <  1:1101774/71‑1 (MQ=255)                                                     tGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTAt                                 >  1:3166325/1‑50 (MQ=255)                                                     tGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTAt                                 >  1:2941588/1‑50 (MQ=255)                                                     tGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTAt                                 >  1:1941421/1‑50 (MQ=255)                                                     tGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTAt                                 >  1:469675/1‑50 (MQ=255)                                                     tGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTAt                                 >  1:1591909/1‑50 (MQ=255)                                                            aTGTATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGc     <  1:3729639/71‑1 (MQ=255)                                                               tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAt  >  1:2944135/1‑71 (MQ=255)                                                               tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAt  >  1:3538000/1‑71 (MQ=255)                                                               tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAt  >  1:2757670/1‑71 (MQ=255)                                                               tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAt  >  1:2654051/1‑71 (MQ=255)                                                               tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAt  >  1:2600943/1‑71 (MQ=255)                                                               tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAt  >  1:3954429/1‑71 (MQ=255)                                                               tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAt  >  1:1602805/1‑71 (MQ=255)                                                               tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAt  >  1:1971090/1‑71 (MQ=255)                                                               tATTCAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAt  >  1:2468585/1‑71 (MQ=255)                                                                   |                                                                   GGTCTGTGTCTTTATTTTTGCCGCACTACTGAAAAAAATGGTCCGGCCCATCTGGGCTAATGTATTTAACTCTGCACTGGCAACAATAACAGCAGCCATTATCTACCTGTACCCTTCTCCACTGGTGTGCAAT  >  W3110S.gb/1775014‑1775146

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑