Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,887,874	T→C	F106L (TTT→CTT)	yeaX →	predicted oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,887,874	0	T	C	82.9%	84.2 / 9.8	35	F106L (TTT→CTT)	yeaX	predicted oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (6/0);  new base C (13/16);  total (19/16)
Fisher's exact test for biased strand distribution p-value = 2.16e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAC  >  W3110S.gb/1887807‑1887932                                                                    |                                                           ttCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTg                                                         <  1:3010991/71‑1 (MQ=255) ttCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTg                                                         <  1:3806097/71‑1 (MQ=255) ttCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTg                                                         <  1:4015207/71‑1 (MQ=255)                tCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAg                                          >  1:2958477/1‑71 (MQ=255)                tCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAg                                          >  1:1631469/1‑71 (MQ=255)                tCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAg                                          >  1:1684394/1‑71 (MQ=255)                              tGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCt                             >  1:3683937/1‑70 (MQ=255)                              tGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTg                            >  1:108915/1‑71 (MQ=255)                              tGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTg                            >  1:3399112/1‑71 (MQ=255)                              tGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTg                            >  1:3971682/1‑71 (MQ=255)                              tGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTg                            >  1:426757/1‑71 (MQ=255)                                ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGtt                          <  1:2768979/71‑1 (MQ=255)                                ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGtt                          <  1:1417186/71‑1 (MQ=255)                                ggCGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGtt                          <  1:979260/71‑1 (MQ=255)                                  cGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTa                        >  1:401433/1‑71 (MQ=255)                                  cGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTa                        <  1:2042994/71‑1 (MQ=255)                                  cGATCGGTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTa                        <  1:757537/71‑1 (MQ=255)                                      cGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTaa                                                   >  1:3958556/1‑40 (MQ=255)                                      cGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTaa                                                   >  1:843634/1‑40 (MQ=255)                                      cGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTaa                                                   >  1:1344/1‑40 (MQ=255)                                      cGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTaa                                                   >  1:3323139/1‑40 (MQ=255)                                      cGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTaa                                                   >  1:2420968/1‑40 (MQ=255)                                      cGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTaa                                                   >  1:137599/1‑40 (MQ=255)                                       ggTTAACGATTTCAACGCCTAATAACCTGCTTGCTCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgcg                   >  1:2642006/1‑71 (MQ=255)                                       ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgcg                   >  1:2524755/1‑71 (MQ=255)                                       ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgcg                   >  1:720504/1‑71 (MQ=255)                                       ggTTAACGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATcgcg                   >  1:1790146/1‑71 (MQ=255)                                            aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcgg              <  1:1880059/71‑1 (MQ=255)                                            aCGATTTCAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcgggcgg              <  1:1478282/71‑1 (MQ=255)                                                   cAACGCCTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTgg        <  1:2666201/70‑1 (MQ=255)                                                        ccTAATAACCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAc  <  1:2871746/71‑1 (MQ=255)                                                            ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcggg                 <  1:1886613/52‑1 (MQ=255)                                                            ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcggg                 <  1:426125/52‑1 (MQ=255)                                                            ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCgcggg                 <  1:739869/52‑1 (MQ=255)                                                            ataaCCTGCTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTGTATCgcggg                 <  1:2009660/52‑1 (MQ=255)                                                                    |                                                           TTCCCGCTTTTTGCATCAGCAGGTAAAAGTGGGCGATCGGTTAACGATTTCAACGCCTAATAACCTGTTTGCGCTAATTCCCTCAGCCAGAAAGCATCTGTTTATCGCGGGCGGTATTGGTATCAC  >  W3110S.gb/1887807‑1887932

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑