BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,927,287	T→C	L45P (CTT→CCT)	yobB →	conserved hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,927,287	0	T	C	100.0%	196.8 / NA	54	L45P (CTT→CCT)	yobB	conserved hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (42/12); total (42/12)

ATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACT  >  W3110S.gb/1927224‑1927347

atcatcTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttcttt                                                        <  1:2380933/70‑1 (MQ=255)

         aGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTTGTTTTTCCATCGCCTTCTTTACTGGGgtgt                                              <  1:80556/71‑1 (MQ=255)

         aGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt                                              <  1:425910/71‑1 (MQ=255)

         aGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt                                              <  1:4082095/71‑1 (MQ=255)

         aGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt                                              <  1:1370414/71‑1 (MQ=255)

          gTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt                                              >  1:1618712/1‑70 (MQ=255)

          gTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt                                              >  1:1636216/1‑70 (MQ=255)

          gTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt                                              >  1:1227842/1‑70 (MQ=255)

          gTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGtgtg                                             <  1:2675080/71‑1 (MQ=255)

          gTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGtgtg                                             <  1:1178708/71‑1 (MQ=255)

           tttGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttcttt                                                        <  1:2912027/59‑1 (MQ=255)

           tttGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttcttt                                                        <  1:3625728/59‑1 (MQ=255)

           tttGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGccttcttt                                                        <  1:3410871/59‑1 (MQ=255)

           tttGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGtgtg                                             <  1:2075443/70‑1 (MQ=255)

             tgtgAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGat                                           <  1:3422994/70‑1 (MQ=255)

                gAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt                                              <  1:1375386/64‑1 (MQ=255)

                    gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                    >  1:1214301/1‑70 (MQ=255)

                    gccgccgccAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                    >  1:158672/1‑70 (MQ=255)

                             aGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCt                          <  1:1541963/71‑1 (MQ=255)

                             aGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCt                          <  1:2715479/71‑1 (MQ=255)

                                 aacaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGgtgt                                              <  1:4098206/47‑1 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTcgc                                    >  1:4030493/1‑56 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACTTGCCCTTcc                       >  1:1195778/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:3546018/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:2441251/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:3786432/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:3021201/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:3992271/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:2715776/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:1007525/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:1430946/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:588423/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:436103/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:1900359/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:429667/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:2712956/1‑69 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTc                        >  1:1563435/1‑68 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCt                      >  1:420742/1‑70 (MQ=255)

                                  acaaTGTCAGCTGTTAGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTg                     >  1:2037478/1‑71 (MQ=255)

                                                 aGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:4048841/1‑54 (MQ=255)

                                                 aGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:3829862/1‑54 (MQ=255)

                                                 aGTTTTTCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTcc                       >  1:2719662/1‑54 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGattatt                                       >  1:3776187/1‑35 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCGCTTCCTGCCCCACCCGATCTCTCAc   >  1:3011986/1‑71 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCt                      >  1:844845/1‑52 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGc                    >  1:2310255/1‑54 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCa    >  1:3980770/1‑70 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCAc   >  1:4054628/1‑71 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCAc   >  1:3187240/1‑71 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCAc   >  1:3117814/1‑71 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCAc   >  1:3113031/1‑71 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCAc   >  1:2832615/1‑71 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCAc   >  1:1815708/1‑71 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCAc   >  1:1091089/1‑71 (MQ=255)

                                                    ttttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTG‑CCTTCCTGCCCCACCCGATCTCTCACt  >  1:4092340/1‑71 (MQ=255)

                                                     tttCCATCGCCTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACt  >  1:1289014/1‑71 (MQ=255)

                                                     tttCCATCGCCTTCTTTACTGGGGTGTGATTATTAGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACt  >  1:1484519/1‑71 (MQ=255)

                                                     tttCCATCGCCTTCTTTACTGGGGTGTGATAATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACt  >  1:3195414/1‑71 (MQ=255)

ATCATCTGGAGTTTGTGAGGGCCGCCGCCAGGCAACAATGTCAGCTGTTAGTTTTTCCATCGCTTTCTTTACTGGGGTGTGATTATTCGCGACGTGCCCTTCCTGCCCCACCCGATCTCTCACT  >  W3110S.gb/1927224‑1927347

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`