| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 230,040 | C→A | R281L (CGC→CTC) | yafC ← | predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 230,040 | 0 | C | A | 93.9% | 85.0 / 1.0 | 33 | R281L (CGC→CTC) | yafC | predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (2/0); new base A (13/18); total (15/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.99e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01 | |||||||||||
TGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCT > W3110S.gb/229978‑230108 | tGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTa < 1:1872123/68‑1 (MQ=255) aCAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTa < 1:1325228/70‑1 (MQ=255) aCAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTa < 1:1349200/70‑1 (MQ=255) aCAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTa < 1:210598/70‑1 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTg > 1:234968/1‑70 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTg > 1:1075365/1‑70 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTg > 1:3659519/1‑70 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTg > 1:3788155/1‑70 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTg > 1:2039201/1‑70 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt < 1:268356/71‑1 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt < 1:2518865/71‑1 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt < 1:1944577/71‑1 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt < 1:1035937/71‑1 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt < 1:1581589/71‑1 (MQ=255) aGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGt < 1:2582043/71‑1 (MQ=255) aGCTGATTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGACGCtc > 1:1407371/1‑69 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTAc > 1:1068307/1‑42 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTAc > 1:2619039/1‑42 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGGACGGTCGCTGTAATAGACGGCACTg < 1:3831630/70‑1 (MQ=255) cTCGCTAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACAGCACGGTCGCTGTAATAGACGGCACTg < 1:868738/70‑1 (MQ=255) tAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:2624974/65‑1 (MQ=255) tAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:3483759/65‑1 (MQ=255) tAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:3676545/65‑1 (MQ=255) tAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:4059543/65‑1 (MQ=255) tAAGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:1733993/65‑1 (MQ=255) aGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGaa > 1:251500/1‑65 (MQ=255) aGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGaa > 1:3675826/1‑65 (MQ=255) aGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGaa > 1:1965808/1‑65 (MQ=255) aGGAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGaa > 1:497870/1‑65 (MQ=255) ggAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTg > 1:2632077/1‑47 (MQ=255) ggAAATCGATAAAAGCCCGGATGAGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTg < 1:233/62‑1 (MQ=255) tGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCt > 1:2498046/1‑71 (MQ=255) tGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCt > 1:2296268/1‑71 (MQ=255) | TGACAGCTCCTCCGGGAGCTGTTTTTACATGCTCGCTAAGGAAATCGATAAAAGCCCGGATGCGCGTACTTACCGCACGGTCGCTGTAATAGACGGCACTGAATGGCATTTCCACTGGCAACACTTTATCT > W3110S.gb/229978‑230108 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |