| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 1,995,272 | T→C | S524S (TCA→TCG) | uvrC ← | excinuclease UvrABC, endonuclease subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 1,995,272 | 0 | T | C | 98.6% | 245.5 / ‑4.2 | 69 | S524S (TCA→TCG) | uvrC | excinuclease UvrABC, endonuclease subunit |
| Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (40/28); total (40/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.20e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.58e-01 | |||||||||||
ACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAC > W3110S.gb/1995206‑1995339 | aCGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGaa < 1:3460796/70‑1 (MQ=255) cccGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTggc < 1:1895154/70‑1 (MQ=255) cccGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTggc < 1:2797983/70‑1 (MQ=255) gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGaa > 1:1519316/1‑52 (MQ=255) gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:2796160/1‑71 (MQ=255) gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:1396763/1‑71 (MQ=255) gTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:704875/1‑71 (MQ=255) tgATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:1726018/1‑71 (MQ=255) tgATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:3677871/1‑71 (MQ=255) tgATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:3976495/1‑71 (MQ=255) aTTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTcc > 1:1739506/1‑71 (MQ=255) aTTCATCGCGAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTc > 1:1161169/1‑70 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:3395305/1‑51 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:1376740/1‑51 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATc > 1:3184129/1‑51 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:3161071/1‑60 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:3296626/1‑60 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:1859358/1‑60 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:2386343/71‑1 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:795391/71‑1 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:2510734/71‑1 (MQ=255) gAATATGCTGGATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:1364761/71‑1 (MQ=255) tatGCTGTATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAg < 1:1824313/68‑1 (MQ=255) ggATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt > 1:3162168/1‑71 (MQ=255) ggATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt > 1:569247/1‑71 (MQ=255) gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:617067/70‑1 (MQ=255) gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:2721017/70‑1 (MQ=255) gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:2416099/70‑1 (MQ=255) gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:356494/70‑1 (MQ=255) gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:3583549/70‑1 (MQ=255) gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:3620564/70‑1 (MQ=255) gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:3886652/70‑1 (MQ=255) gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:904355/70‑1 (MQ=255) gATAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt < 1:3839117/70‑1 (MQ=255) tAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:922428/1‑70 (MQ=255) tAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:2581769/1‑70 (MQ=255) tAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:845316/1‑70 (MQ=255) tAACATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:2255935/1‑70 (MQ=255) aCATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGttta > 1:1976630/1‑67 (MQ=255) aCATGCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtc > 1:2480811/1‑68 (MQ=255) gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt > 1:4082800/1‑62 (MQ=255) gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGtt > 1:3062720/1‑62 (MQ=255) gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:3148894/1‑69 (MQ=255) gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:2348827/1‑69 (MQ=255) gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:2076821/1‑69 (MQ=255) gCAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:1722489/1‑69 (MQ=255) gCAGCGAGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:5621/1‑69 (MQ=255) cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtcca > 1:2248382/1‑65 (MQ=255) cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtcca > 1:1709822/1‑65 (MQ=255) cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtcca > 1:231827/1‑65 (MQ=255) cAGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtcca > 1:2864977/1‑65 (MQ=255) aGCGCGGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGc < 1:1558867/71‑1 (MQ=255) gcgGGCTAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:3802766/71‑1 (MQ=255) gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:880130/71‑1 (MQ=255) gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:1382140/71‑1 (MQ=255) gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:1058528/71‑1 (MQ=255) gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:3733678/71‑1 (MQ=255) gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:2792076/71‑1 (MQ=255) gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:2531389/71‑1 (MQ=255) gcgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTAAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:1457946/71‑1 (MQ=255) cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCtt > 1:2686891/1‑36 (MQ=255) cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:32317/1‑63 (MQ=255) cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:2601467/1‑63 (MQ=255) cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:2459122/1‑63 (MQ=255) cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagtc > 1:988207/1‑63 (MQ=255) cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTtccagt > 1:1263049/1‑62 (MQ=255) cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:2812295/70‑1 (MQ=255) cgGGCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCtt < 1:1014484/70‑1 (MQ=255) gggCGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAc < 1:1655103/71‑1 (MQ=255) | ACGGTGCCCGCCAATCGCGTGATCATGTGATTCATCGCGAATATGCTGGATAACATGCAGCGCGGGTGAATCTGGCGGCAAACTAAATCCCTCACCTTCCGGCTCAAAGAACAGCGTTTCCAGTCCAGCCTTAC > W3110S.gb/1995206‑1995339 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |