BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,006,018	A→G	I4V (ATT→GTT)	fliD →	flagellar filament capping protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,006,018	0	A	G	100.0%	116.1 / NA	32	I4V (ATT→GTT)	fliD	flagellar filament capping protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (11/21); total (11/21)

ATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGC  >  W3110S.gb/2005952‑2006079

aTTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTc                                                           <  1:3784654/71‑1 (MQ=255)

aTTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTc                                                           <  1:1865140/71‑1 (MQ=255)

aTTCAGACCGATAACCCAGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTc                                                           <  1:1282363/71‑1 (MQ=255)

           tAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAg                                                <  1:594342/71‑1 (MQ=255)

           tAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAg                                                <  1:265042/71‑1 (MQ=255)

                ccGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCggg                                           >  1:2729369/1‑71 (MQ=255)

                    tATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAg                                       <  1:547542/71‑1 (MQ=255)

                         gTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTg                                  <  1:703795/71‑1 (MQ=255)

                           tttACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGttt                                                            >  1:851397/1‑43 (MQ=255)

                           tttACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGttt                                                            >  1:2169291/1‑43 (MQ=255)

                            ttACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGa                                <  1:1757554/70‑1 (MQ=255)

                            ttACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGa                                <  1:2252115/70‑1 (MQ=255)

                            ttACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGa                                <  1:142820/70‑1 (MQ=255)

                            ttACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGa                                <  1:444039/70‑1 (MQ=255)

                            ttACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGa                                <  1:3598057/70‑1 (MQ=255)

                               cGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGAttt                             <  1:1464179/70‑1 (MQ=255)

                                        aGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATc                   <  1:1879056/71‑1 (MQ=255)

                                        aGATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATc                   <  1:1858528/71‑1 (MQ=255)

                                         gATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGt                         <  1:1310193/64‑1 (MQ=255)

                                         gATAAAAGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGt                         <  1:235811/64‑1 (MQ=255)

                                               aGGAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCAATCCTTGATa            <  1:2933182/71‑1 (MQ=255)

                                                 gAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGc          <  1:2741636/71‑1 (MQ=255)

                                                 gAAATCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGc          <  1:2332535/71‑1 (MQ=255)

                                                  aaaTCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGcc         >  1:3070088/1‑71 (MQ=255)

                                                  aaaTCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGcc         >  1:128956/1‑71 (MQ=255)

                                                  aaaTCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGcc         >  1:1222699/1‑71 (MQ=255)

                                                  aaaTCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGc          >  1:1601613/1‑70 (MQ=255)

                                                  aaaTCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGc          >  1:484329/1‑70 (MQ=255)

                                                   aaTCGCATGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCt        <  1:1488456/71‑1 (MQ=255)

                                                         aTGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAccgc  >  1:2766898/1‑71 (MQ=255)

                                                         aTGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAccgc  >  1:1612241/1‑71 (MQ=255)

                                                         aTGGCAAGTGTTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAccgc  >  1:716906/1‑71 (MQ=255)

ATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGC  >  W3110S.gb/2005952‑2006079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`