| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,077,980 | C→T | Y355Y (TAC→TAT) | yeeR → | predicted membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,077,980 | 0 | C | T | 90.6% | 185.1 / 8.1 | 64 | Y355Y (TAC→TAT) | yeeR | predicted membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (0/6); new base T (36/22); total (36/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.02e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTAC > W3110S.gb/2077916‑2078040 | cctcctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGctcc > 1:2638885/1‑71 (MQ=255)cctcctGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGctcc > 1:2232903/1‑71 (MQ=255) cAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3686316/70‑1 (MQ=255) cAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3100583/70‑1 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:3558037/1‑70 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:2391294/1‑70 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:450681/70‑1 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:2327978/1‑70 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:4024492/70‑1 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:1732856/70‑1 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:36023/70‑1 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:1589124/1‑70 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:3131122/70‑1 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:1360933/70‑1 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa < 1:3135855/70‑1 (MQ=255) catcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCgaaacagaaa > 1:3559169/1‑70 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:1894500/1‑57 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:162248/1‑57 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:2002752/1‑57 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:428017/1‑57 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:1380273/1‑57 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTc > 1:2956861/1‑56 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAacac < 1:3459303/71‑1 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAacac < 1:567577/71‑1 (MQ=255) tcatGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTACTCTCTTTGTcg > 1:2490892/1‑57 (MQ=255) tcatGCCCCGGAAAATCATAAG‑‑GGATCCGGAATGGTATTGCTCCTCTCTTTGTcg > 1:2663035/1‑55 (MQ=255) cgccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:883182/3‑71 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgtg > 1:851855/1‑70 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgtg > 1:1297373/1‑70 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACtgtg > 1:1260480/1‑70 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:1999962/1‑71 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:3712006/1‑71 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:1325611/1‑71 (MQ=255) ccccGGAAAATCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTgtgt > 1:314778/1‑71 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3823589/47‑1 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:647175/47‑1 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:709581/47‑1 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:816056/47‑1 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3125874/47‑1 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:3314045/47‑1 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:2950994/47‑1 (MQ=255) aaTCATAAGCCGGATCCGGAATGGTATTGCTCCTCTCTTTGTCGCga < 1:1945499/47‑1 (MQ=255) aTCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt < 1:1011573/71‑1 (MQ=255) aTCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt < 1:3939035/71‑1 (MQ=255) aTCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt < 1:2077821/71‑1 (MQ=255) tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGa > 1:163348/1‑63 (MQ=255) tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:3430607/1‑70 (MQ=255) tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:3794979/1‑70 (MQ=255) tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:2806011/1‑70 (MQ=255) tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:696447/1‑70 (MQ=255) tCCGGAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCt > 1:2386811/1‑70 (MQ=255) ggAATGGTATTGCTCCTCTCTTTGTCGCgaaacag > 1:309151/1‑35 (MQ=255) ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:2701629/1‑70 (MQ=255) ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:1499525/1‑70 (MQ=255) ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:464125/1‑70 (MQ=255) ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:412991/1‑70 (MQ=255) ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:3896334/1‑70 (MQ=255) ggAATGGTATTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTac > 1:3664147/1‑70 (MQ=255) tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGaaagt < 1:1971043/51‑3 (MQ=255) tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:2645054/51‑1 (MQ=255) tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:3448319/51‑1 (MQ=255) tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:1951754/51‑1 (MQ=255) tGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:3393240/51‑1 (MQ=255) ggTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAAtt < 1:900873/50‑1 (MQ=255) | CCTCCTGCGGGCTGACAGAAGTACATCATGCCCCGGAAAATCATAAGCCGGATCCGGAATGGTACTGCTCCTCTCTTTGTCGCGAAACAGAAACACTGTGTCAGGAAATTTATGAACGCCCTTAC > W3110S.gb/2077916‑2078040 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |