Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,157,782	T→C	L128S (TTG→TCG)	mdtB →	multidrug efflux system, subunit B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,157,782	0	T	C	100.0%	43.8 / NA	13	L128S (TTG→TCG)	mdtB	multidrug efflux system, subunit B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/0);  total (13/0)

CGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAC  >  W3110S.gb/2157735‑2157825                                                |                                            cGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACccgc                      >  1:2999812/1‑71 (MQ=255) cGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACccgc                      >  1:796076/1‑71 (MQ=255) cGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACccgc                      >  1:849273/1‑71 (MQ=255) cGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACccgc                      >  1:918633/1‑71 (MQ=255)               tGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCa          >  1:723141/1‑69 (MQ=255)               tGCAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCa          >  1:922815/1‑69 (MQ=255)                 cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCgg                   >  1:2141826/1‑58 (MQ=255)                 cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGt      >  1:1152395/1‑71 (MQ=255)                 cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGt      >  1:2412310/1‑71 (MQ=255)                 cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGt      >  1:3112463/1‑71 (MQ=255)                 cAGGCCGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGt      >  1:3815944/1‑71 (MQ=255)                     ccGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAc  >  1:13867/1‑71 (MQ=255)                     ccGCGATTAACGCTGCGACCAACTTGTCGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAc  >  1:918327/1‑71 (MQ=255)                                                |                                            CGCCGAGCAGGAAGTGCAGGCCGCGATTAACGCTGCGACCAACTTGTTGCCGAGCGATCTGCCTAACCCGCCGGTTTACAGCAAAGTGAAC  >  W3110S.gb/2157735‑2157825

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑