Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,160,543	C→T	F7F (TTC→TTT)	mdtC →	multidrug efflux system, subunit C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,160,543	0	C	T	100.0%	78.6 / NA	26	F7F (TTC→TTT)	mdtC	multidrug efflux system, subunit C
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (12/14);  total (12/14)

CCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCA  >  W3110S.gb/2160485‑2160598                                                           |                                                        ccAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCg                                             >  1:464346/1‑71 (MQ=255) ccAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCg                                             >  1:3536213/1‑71 (MQ=255) ccAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCg                                             >  1:3117214/1‑71 (MQ=255)     aaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTgg                                         >  1:1017469/1‑71 (MQ=255)     aaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTgg                                         >  1:1965504/1‑71 (MQ=255)     aaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTgg                                         >  1:3938796/1‑71 (MQ=255)     aaGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTgg                                         >  1:3784239/1‑71 (MQ=255)      aGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGc                                        <  1:950056/71‑1 (MQ=255)      aGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGc                                        <  1:3991650/71‑1 (MQ=255)      aGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGc                                        <  1:2243492/71‑1 (MQ=255)      aGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGc                                        <  1:3782551/71‑1 (MQ=255)           cTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGcgacga                                   <  1:1068120/71‑1 (MQ=255)                cccGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTAtt                                                      >  1:2311137/1‑47 (MQ=255)                          agagGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTg                    <  1:2232606/71‑1 (MQ=255)                          agagGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTg                    <  1:1978018/71‑1 (MQ=255)                          agagGAGGCGTAAGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTg                    <  1:778121/71‑1 (MQ=255)                                      aGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCtgt        >  1:3246665/1‑71 (MQ=255)                                      aGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCtg         >  1:1601018/1‑70 (MQ=255)                                      aGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACACtgt        >  1:2477920/1‑71 (MQ=255)                                      aGTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTAATGTCGGTTGCAATTACCCtgt        >  1:3164784/1‑71 (MQ=255)                                       gTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGcgacga                                   <  1:3295357/43‑1 (MQ=255)                                       gTGAAGTTTTTTGCCCTCTTTATTTACCGCCCGGTGGcgacga                                   <  1:464655/43‑1 (MQ=255)                                            gTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCa  <  1:3515406/71‑1 (MQ=255)                                            gTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCa  <  1:2405995/71‑1 (MQ=255)                                            gTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCa  <  1:404714/71‑1 (MQ=255)                                            gTTTTTTGCCCTCTTTATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCa  <  1:1774197/71‑1 (MQ=255)                                                           |                                                        CCAAAAGCCGCTTTGCCCGTCATGAAGAGGAGGCGTAAGTGAAGTTTTTTGCCCTCTTCATTTACCGCCCGGTGGCGACGATTTTACTGTCGGTTGCCATTACCCTGTGCGGCA  >  W3110S.gb/2160485‑2160598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑