Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	234,234	C→T	L183L (TTG→TTA)	gloB ←	predicted hydroxyacylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	234,234	0	C	T	100.0%	245.6 / NA	79	L183L (TTG→TTA)	gloB	predicted hydroxyacylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (25/54);  total (25/54)

TTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCACAA  >  W3110S.gb/234179‑234297                                                        |                                                                ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag                                                   <  1:2001734/70‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag                                                   <  1:1527617/70‑1 (MQ=255) ttATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaag                                                   <  1:128770/70‑1 (MQ=255)   aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa                                                 >  1:4061142/1‑70 (MQ=255)   aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa                                                 >  1:2337163/1‑70 (MQ=255)   aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa                                                 >  1:140891/1‑70 (MQ=255)   aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa                                                 >  1:2786921/1‑70 (MQ=255)   aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa                                                 >  1:3629012/1‑70 (MQ=255)   aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa                                                 >  1:3850930/1‑70 (MQ=255)   aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa                                                 >  1:3537034/1‑70 (MQ=255)   aTTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGgaagaa                                                 >  1:2675161/1‑70 (MQ=255)    tttGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAAt                                                <  1:1465431/70‑1 (MQ=255)    tttGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAAt                                                <  1:2033736/70‑1 (MQ=255)      tGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACt                                             >  1:2678102/1‑71 (MQ=255)      tGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACt                                             >  1:4006328/1‑71 (MQ=255)      tGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACt                                             >  1:2418247/1‑71 (MQ=255)      tGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACt                                             >  1:529716/1‑71 (MQ=255)      tGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACt                                             >  1:1724296/1‑71 (MQ=255)      tGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACt                                             >  1:1729284/1‑71 (MQ=255)      tGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACt                                             >  1:3565771/1‑71 (MQ=255)         ttttttGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCaa                                          <  1:3185521/71‑1 (MQ=255)         ttttttGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCaa                                          <  1:788637/71‑1 (MQ=255)                cccGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAAc                                   <  1:2856898/71‑1 (MQ=255)                cccGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAAc                                   <  1:2316470/71‑1 (MQ=255)                cccGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAAc                                   <  1:4110510/71‑1 (MQ=255)                cccGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAAc                                   <  1:4078504/71‑1 (MQ=255)                cccGTAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAAc                                   <  1:546286/71‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGTATAAATCGTGCGGAAGAATa                                               <  1:2722450/55‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGTATAAATCGTGCGGAAGAATACTCAAAGCaaa                                    <  1:399844/66‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa                                               <  1:3163800/55‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa                                               <  1:1644186/55‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa                                               <  1:2386934/55‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa                                               <  1:797558/55‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa                                               <  1:1254707/55‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCaaa                                    <  1:2447873/66‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCaaa                                    <  1:3824961/66‑1 (MQ=255)                    tAACTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCaaa                                    <  1:1118203/66‑1 (MQ=255)                      aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata                             >  1:2239921/1‑71 (MQ=255)                      aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata                             <  1:3263387/71‑1 (MQ=255)                      aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata                             <  1:3293959/71‑1 (MQ=255)                      aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata                             <  1:434852/71‑1 (MQ=255)                      aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata                             <  1:1655781/71‑1 (MQ=255)                      aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata                             <  1:1293194/71‑1 (MQ=255)                      aCTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCata                             <  1:1221480/71‑1 (MQ=255)                       cTCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCat                              <  1:3185428/69‑1 (MQ=255)                        tCCTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATa                                               <  1:301503/51‑1 (MQ=255)                          cTTAACTTTACGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACtt                                 <  1:3905432/63‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATATATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:904627/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:2781129/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:3796540/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:3734154/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:90487/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:94892/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:182046/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:1183048/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:1256102/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:2096057/70‑1 (MQ=255)                                   aCGATAATAATCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGta                 <  1:2778495/70‑1 (MQ=255)                                          taatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATg         <  1:856328/71‑1 (MQ=255)                                          taatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATg         <  1:689834/71‑1 (MQ=255)                                          taatCATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATg         <  1:212531/71‑1 (MQ=255)                                              cATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAAc                                   >  1:1550801/1‑41 (MQ=255)                                              cATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACt                                  >  1:1781597/1‑42 (MQ=255)                                              cATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACt                                  >  1:1289649/1‑42 (MQ=255)                                              cATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACt                                  >  1:819241/1‑42 (MQ=255)                                              cATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACt                                  >  1:348624/1‑42 (MQ=255)                                              cATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACt                                  >  1:3529996/1‑42 (MQ=255)                                              cATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACt                                  >  1:405239/1‑42 (MQ=255)                                              cATTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACt                                  >  1:2739366/1‑42 (MQ=255)                                              aaTTTATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACt                                  >  1:335882/2‑42 (MQ=255)                                                tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca   <  1:2193214/71‑1 (MQ=255)                                                tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca   <  1:2350524/71‑1 (MQ=255)                                                tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca   <  1:135336/71‑1 (MQ=255)                                                tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca   <  1:814438/71‑1 (MQ=255)                                                tttATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGcaca   <  1:2599987/71‑1 (MQ=255)                                                  tATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa  <  1:1626470/70‑1 (MQ=255)                                                  tATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa  <  1:367362/70‑1 (MQ=255)                                                  tATGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa  <  1:3840477/70‑1 (MQ=255)                                                    tGGATAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCacaa  <  1:1443808/68‑1 (MQ=255)                                                        |                                                                TTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATCGTGCGGAAGAATACTCAAAGCAAACTTCATATTTGATAAGGTATATTCATGAGCACAA  >  W3110S.gb/234179‑234297

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑