Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 2,198,825 G→A A398T (GCG→ACG)  metG → methionyl‑tRNA synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb2,198,8250GA98.6% 209.5 / ‑4.0 70A398T (GCG→ACG) metGmethionyl‑tRNA synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (54/15);  minor base T (1/0);  total (55/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

CGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACA  >  W3110S.gb/2198767‑2198891
                                                          |                                                                  
cGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATc                                                         <  1:3076697/70‑1 (MQ=255)
cGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATc                                                         <  1:957731/70‑1 (MQ=255)
cGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATc                                                         <  1:3541149/70‑1 (MQ=255)
cGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATc                                                         <  1:3053835/70‑1 (MQ=255)
cGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATc                                                         <  1:1720621/70‑1 (MQ=255)
       cGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCg                                                 >  1:3518398/1‑71 (MQ=255)
       cGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCg                                                 >  1:3774261/1‑71 (MQ=255)
       cGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCg                                                 >  1:2111062/1‑71 (MQ=255)
       cGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCg                                                 >  1:3904877/1‑71 (MQ=255)
         tgtgAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGtt                                               <  1:2863684/71‑1 (MQ=255)
          gtgAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTa                                                           >  1:2349644/1‑58 (MQ=255)
          gtgAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:3156077/1‑71 (MQ=255)
          gtgAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:2522253/1‑71 (MQ=255)
          gtgAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:2337349/1‑71 (MQ=255)
             aaTGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGa                                           >  1:381391/1‑71 (MQ=255)
                gCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACgg                                        >  1:1245283/1‑71 (MQ=255)
                 ccGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGc                                       >  1:3499963/1‑71 (MQ=255)
                  cGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTTACGGCg                                      <  1:3639/71‑1 (MQ=255)
                  cGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCg                                      <  1:3777160/71‑1 (MQ=255)
                  cGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCg                                      <  1:2108485/71‑1 (MQ=255)
                  cGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCg                                      <  1:1872648/71‑1 (MQ=255)
                  cGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCg                                      <  1:3937564/71‑1 (MQ=255)
                  cGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCg                                      <  1:4039810/71‑1 (MQ=255)
                  cGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCg                                      <  1:2375687/71‑1 (MQ=255)
                  cGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCg                                      <  1:1341388/71‑1 (MQ=255)
                  cGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCg                                      <  1:1325277/71‑1 (MQ=255)
                         gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg                                >  1:65151/1‑70 (MQ=255)
                         gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg                                >  1:3617098/1‑70 (MQ=255)
                         gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg                                >  1:740620/1‑70 (MQ=255)
                         gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg                                >  1:3134469/1‑70 (MQ=255)
                         gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg                                >  1:1253508/1‑70 (MQ=255)
                         gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg                                >  1:2796358/1‑70 (MQ=255)
                         gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg                                >  1:1882612/1‑70 (MQ=255)
                         gTTAACAAAGTGGTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTgg                                >  1:2340112/1‑70 (MQ=255)
                                     gTTAACCTGGCCTCCCGTAATTCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGc                   >  1:4024324/1‑71 (MQ=255)
                                     gTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGc                   >  1:677757/1‑71 (MQ=255)
                                     gTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGc                   >  1:3949363/1‑71 (MQ=255)
                                     gTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGc                   >  1:603726/1‑71 (MQ=255)
                                     gTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGc                   >  1:1594907/1‑71 (MQ=255)
                                     gTTAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGc                   >  1:1307610/1‑71 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGtttt                                             >  1:771570/1‑43 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGtttt                                             >  1:3418013/1‑43 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGtttt                                             >  1:2601502/1‑43 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGtttt                                             >  1:2630704/1‑43 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGtttt                                             >  1:549072/1‑43 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:701440/1‑42 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:3382773/1‑42 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:85438/1‑42 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:1112556/1‑42 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:4014742/1‑42 (MQ=255)
                                       tAACCTGGCCTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:2731058/1‑42 (MQ=255)
                                       tAACCTGGACTCCCGTAATACGGGCTTTATCAACAAGCGttt                                              >  1:35117/1‑42 (MQ=38)
                                                cTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt        >  1:1093212/1‑71 (MQ=255)
                                                cTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt        >  1:4132276/1‑71 (MQ=255)
                                                cTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt        >  1:4087859/1‑71 (MQ=255)
                                                cTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt        >  1:1647687/1‑71 (MQ=255)
                                                cTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt        >  1:1184062/1‑71 (MQ=255)
                                                cTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt        >  1:2540457/1‑71 (MQ=255)
                                                cTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt        >  1:1015177/1‑71 (MQ=255)
                                                cTCCCGTAATACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGt        >  1:271483/1‑71 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGc                                   >  1:3458538/1‑37 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGAcc              >  1:601060/1‑58 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa  >  1:1897206/1‑70 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa  >  1:1995241/1‑70 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa  >  1:4138051/1‑70 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa  >  1:2226691/1‑70 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa  >  1:3773465/1‑70 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa  >  1:2370399/1‑70 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa  >  1:1102104/1‑70 (MQ=255)
                                                       aaTACGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACa  >  1:2594267/1‑70 (MQ=255)
                                                          |                                                                  
CGTTCAGCGTGTGAATGCCGATATCGTTAACAAAGTGGTTAACCTGGCCTCCCGTAATGCGGGCTTTATCAACAAGCGTTTTGACGGCGTGCTGGCAAGCGAACTGGCTGACCCGCAGTTGTACA  >  W3110S.gb/2198767‑2198891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: