| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,233,079 | G→A | A214V (GCG→GTG) | dusC ← | tRNA‑dihydrouridine synthase C |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,233,079 | 0 | G | A | 91.9% | 99.2 / 1.5 | 37 | A214V (GCG→GTG) | dusC | tRNA‑dihydrouridine synthase C |
| Reads supporting (aligned to +/- strand): ref base G (3/0); new base A (13/21); total (16/21) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.21e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01 | |||||||||||
CACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCG > W3110S.gb/2233012‑2233133 | caccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCa < 1:1006137/71‑1 (MQ=255)caccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCa < 1:1001356/71‑1 (MQ=255)caccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCa < 1:137763/71‑1 (MQ=255)caccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCa < 1:1752161/71‑1 (MQ=255)caccCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCa < 1:1706973/71‑1 (MQ=255) tcTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCAtt < 1:1845451/70‑1 (MQ=255) gCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCAtt < 1:1124674/71‑1 (MQ=255) gCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCAtt < 1:2601717/71‑1 (MQ=255) gggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTgcgcgc > 1:4061357/1‑71 (MQ=255) gggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTgcgcg > 1:2195279/1‑70 (MQ=255) gggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCATTGTTgcgcg < 1:1794563/70‑1 (MQ=255) gggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCATTGTTgcgcg < 1:1529208/70‑1 (MQ=255) gggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCATTGTTgcgcg < 1:1970039/70‑1 (MQ=255) gggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCATTGTTgcgcg < 1:2891525/70‑1 (MQ=255) gggAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCATTGTTgcgcg < 1:1223740/70‑1 (MQ=255) tatTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGc < 1:1315857/71‑1 (MQ=255) atTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGc < 1:259202/70‑1 (MQ=255) cgACCAATCATCACTGCGTCGCAGCCGCTGATCACCa < 1:1635207/37‑1 (MQ=255) aaTCATCACTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGc < 1:1619090/52‑1 (MQ=255) atcatcACTTCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGtt < 1:2187247/71‑1 (MQ=255) atcatcACTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGtt < 1:478548/71‑1 (MQ=255) atcatcACTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGtt < 1:3840713/71‑1 (MQ=255) atcatcACTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGtt < 1:3007811/71‑1 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGTGCGCTCTGCCAGTCCCAGATTTCa > 1:4112270/1‑61 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGAtttaa > 1:244785/1‑59 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCa > 1:2716858/1‑61 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCa > 1:3167199/1‑61 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCa > 1:3413519/1‑61 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCa > 1:3569462/1‑61 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCa > 1:2147631/1‑61 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCa > 1:40036/1‑61 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCa > 1:1097833/1‑61 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCa > 1:472329/1‑61 (MQ=255) caCTGCGTCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCAAGATTTCa > 1:2876333/1‑61 (MQ=255) aCTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAAt > 1:1928998/1‑71 (MQ=255) tCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCaa > 1:430892/1‑64 (MQ=255) tCGCAGCCGCTGATCACCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCg > 1:2210989/1‑70 (MQ=255) | CACCCGGCTCAGGTTGGGAATATTGAGCGCCCCGCGACCAATCATCACTGCGTCGCAGCCGCTGATCGCCATGCATTGTTGCGCGCTCTGCCAGTCCCAGATTTCACCGTTGGCAATCACCG > W3110S.gb/2233012‑2233133 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |