Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,258,347	C→T	E58E (GAG→GAA)	rihB ←	ribonucleoside hydrolase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,258,347	0	C	T	100.0%	131.1 / NA	42	E58E (GAG→GAA)	rihB	ribonucleoside hydrolase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (26/16);  total (26/16)

GGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAC  >  W3110S.gb/2258287‑2258405                                                             |                                                           ggCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCt                                                  <  1:941123/71‑1 (MQ=255) ggCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCt                                                  <  1:3137175/71‑1 (MQ=255)       gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa                                            >  1:562668/1‑71 (MQ=255)       gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa                                            >  1:2571194/1‑71 (MQ=255)       gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa                                            >  1:1852782/1‑71 (MQ=255)       gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa                                            >  1:3742798/1‑71 (MQ=255)       gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa                                            >  1:3737979/1‑71 (MQ=255)       gATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa                                            >  1:3185774/1‑71 (MQ=255)               gACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAgg                                    >  1:1246461/1‑71 (MQ=255)               gACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAgg                                    >  1:1436108/1‑71 (MQ=255)               gACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAgg                                    >  1:2349398/1‑71 (MQ=255)               gACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg                                     <  1:3079906/70‑1 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg                                     <  1:292610/49‑1 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg                                     <  1:3291283/49‑1 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg                                     <  1:1980619/49‑1 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAg                                     <  1:344159/49‑1 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa                                 >  1:4134166/1‑53 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa                                 >  1:2961817/1‑53 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa                                 >  1:1475226/1‑53 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa                                 >  1:1842083/1‑53 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa                                 >  1:1935809/1‑53 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa                                 >  1:19861/1‑53 (MQ=255)                                    tCCCCGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCa                                 >  1:1962909/1‑53 (MQ=255)                                        cGCATAAACCGGAACATTAATTTCCAGTTTCTGTCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCg           <  1:2020097/71‑1 (MQ=255)                                        cGCATAAACCGGAACATTAATTTCCAGTTTCTGGCaaa                                            >  1:3580221/1‑38 (MQ=255)                                        cGCATAAACCGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCg           <  1:2624398/71‑1 (MQ=255)                                                cctgAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa   <  1:2290823/68‑1 (MQ=255)                                                ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa   <  1:69315/71‑1 (MQ=255)                                                ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa   <  1:3642112/71‑1 (MQ=255)                                                ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa   <  1:2625748/71‑1 (MQ=255)                                                ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa   <  1:3237399/71‑1 (MQ=255)                                                ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa   <  1:3131434/71‑1 (MQ=255)                                                ccGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTa   <  1:296448/71‑1 (MQ=255)                                                 cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATg                       >  1:927065/1‑50 (MQ=255)                                                 cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGt                      >  1:1914584/1‑51 (MQ=255)                                                 cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc  >  1:866858/1‑71 (MQ=255)                                                 cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc  >  1:1237620/1‑71 (MQ=255)                                                 cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc  >  1:3563960/1‑71 (MQ=255)                                                 cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc  >  1:852641/1‑71 (MQ=255)                                                 cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc  >  1:2421159/1‑71 (MQ=255)                                                 cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc  >  1:1089178/1‑71 (MQ=255)                                                 cGGAACATTAATTTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAc  >  1:2826306/1‑71 (MQ=255)                                                             |                                                           GGCAACGATTTGTTGACGCATAATGGGCTGCGGCATCCCCGCATAAACCGGAACATTAATCTCCAGTTTCTGGCAAACATTCAGGCCATTAATTAATGTTTTATCAAGCGTCTGATTAC  >  W3110S.gb/2258287‑2258405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑