| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,313,823 | T→C | E349G (GAA→GGA) | yojL ← | predicted thiamine biosynthesis lipoprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,313,823 | 0 | T | C | 95.5% | 232.2 / 0.7 | 67 | E349G (GAA→GGA) | yojL | predicted thiamine biosynthesis lipoprotein |
| Reads supporting (aligned to +/- strand): ref base T (0/3); new base C (38/26); total (38/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.63e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.93e-01 | |||||||||||
ATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATC > W3110S.gb/2313760‑2313875 | aTAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa > 1:4017693/1‑70 (MQ=255)aTAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa > 1:1856215/1‑70 (MQ=255) tAGGCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa > 1:3749376/1‑69 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa > 1:349748/1‑69 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa > 1:3899405/1‑69 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa > 1:1879617/1‑69 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa > 1:570683/1‑69 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGa > 1:28531/1‑69 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc > 1:1743476/1‑70 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc > 1:321256/1‑70 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc > 1:1754224/1‑70 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc > 1:712750/1‑70 (MQ=255) tAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGAc > 1:1384873/1‑70 (MQ=255) tttAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAg < 1:2838663/68‑1 (MQ=255) tttAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAg < 1:225277/68‑1 (MQ=255) tttAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAg < 1:1456856/68‑1 (MQ=255) tAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAg < 1:438777/70‑1 (MQ=255) tAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAg < 1:533845/70‑1 (MQ=255) tAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAg < 1:276161/70‑1 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCtttt > 1:2467753/1‑70 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCtttt > 1:2132426/1‑70 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCtttt > 1:2020674/1‑70 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCtttt > 1:359598/1‑70 (MQ=255) tGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCttt > 1:1317065/1‑69 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt > 1:2648996/1‑71 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt > 1:3744469/1‑71 (MQ=255) gTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt > 1:255575/1‑71 (MQ=255) caccaccacGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACt > 1:1778574/1‑67 (MQ=255) cACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACt > 1:2712625/1‑67 (MQ=255) cACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACt > 1:3275936/1‑67 (MQ=255) cACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACt > 1:3426351/1‑67 (MQ=255) cACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACt > 1:2007420/1‑67 (MQ=255) cACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTgtgg > 1:1297261/1‑71 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa < 1:3899835/70‑1 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa < 1:506332/70‑1 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa < 1:1670790/70‑1 (MQ=255) aTCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGa < 1:1632763/70‑1 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACAt > 1:177243/1‑71 (MQ=255) cACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACAt > 1:591726/1‑71 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACa > 1:2541622/1‑69 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACa > 1:2110415/1‑69 (MQ=255) aCGCAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATc > 1:1327770/1‑71 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:672841/59‑1 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:845036/59‑1 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:1200915/59‑1 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:3264217/59‑1 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:1008183/59‑1 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:2385583/59‑1 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:1825348/59‑1 (MQ=255) cAAAAACCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:2180340/59‑1 (MQ=255) aaaaaCCAACAATCTTGCGCTTTTTTTTTTTCCGCTGACAAGGAAGGTTTTAAACtgt < 1:659988/58‑1 (MQ=255) aaaaCCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGt < 1:2768638/71‑1 (MQ=255) aCCAACAATCTTGCGCTTTAATTTTTTCCGCTGCCAAGGAAGCTTTTAAACTGTGGTGACATCCAGGttt > 1:2144844/1‑70 (MQ=255) aCCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGttt > 1:3598901/1‑70 (MQ=255) aCCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGttt > 1:1675980/1‑70 (MQ=255) aCCAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGttt > 1:2210206/1‑70 (MQ=255) ccAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTa < 1:2983026/71‑1 (MQ=255) ccAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTa < 1:2789387/71‑1 (MQ=255) ccAACAATCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTa < 1:4090692/71‑1 (MQ=255) caaTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACtgt < 1:3099054/49‑1 (MQ=255) aaTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCt < 1:2384441/71‑1 (MQ=255) aaTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCt < 1:2769612/71‑1 (MQ=255) aaTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCt < 1:3634693/71‑1 (MQ=255) aaTCTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCt < 1:914645/71‑1 (MQ=255) cTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATc > 1:1703970/1‑71 (MQ=255) cTTGCGCTTTAATTTTTTCCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATc > 1:1553019/1‑71 (MQ=255) tttAATTTTTTCGGCTGACAAGGAAGCTTTTAAACtgt < 1:3930158/38‑1 (MQ=38) | ATAGCCTTTAGGCTGCCCGGTCACCATCACGCAAAAACCAACAATCTTGCGCTTTAATTTTTTTCGCTGACAAGGAAGCTTTTAAACTGTGGTGACATCCAGGTTTTAAAGCTATC > W3110S.gb/2313760‑2313875 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |