Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,315,568	A→G	G172G (GGT→GGC)	ompC ←	outer membrane porin protein C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,315,568	0	A	G	100.0%	40.4 / NA	13	G172G (GGT→GGC)	ompC	outer membrane porin protein C
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/3);  total (10/3)

CGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCG  >  W3110S.gb/2315507‑2315610                                                              |                                           cGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTa                                    <  1:2626168/70‑1 (MQ=255)   tCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACt                                  >  1:1481459/1‑70 (MQ=255)   tCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACt                                  >  1:2043168/1‑70 (MQ=255)   tCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACt                                  >  1:2072768/1‑70 (MQ=255)   tCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACt                                  >  1:3089508/1‑70 (MQ=255)   tCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACt                                  >  1:3748838/1‑70 (MQ=255)                tagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGtt                     >  1:3063639/1‑70 (MQ=255)                tagttaCGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGtt                     >  1:539457/1‑70 (MQ=255)                                  aGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg  <  1:1411739/71‑1 (MQ=255)                                  aGCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg  <  1:2840269/71‑1 (MQ=255)                                   gCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg  >  1:2059686/1‑70 (MQ=255)                                   gCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg  >  1:2085335/1‑70 (MQ=255)                                   gCCTTCACCAGATGGGTTGCCGTTTTTGCCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCg  >  1:2770193/1‑70 (MQ=255)                                                              |                                           CGTCACGACCGTTGTTAGTTACGCCACTAGTAAAGCCTTCACCAGATGGGTTGCCGTTTTTACCCTGGTACTGAACAGCAAAGTTCAGGCCGTCAACCAGACCG  >  W3110S.gb/2315507‑2315610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑