| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,325,916 | A→G | N402D (AAT→GAT) | atoS → | sensory histidine kinase in two‑component regulatory system with AtoC |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,325,916 | 0 | A | G | 83.0% | 144.5 / 21.6 | 60 | N402D (AAT→GAT) | atoS | sensory histidine kinase in two‑component regulatory system with AtoC |
| Reads supporting (aligned to +/- strand): ref base A (10/0); new base G (19/30); total (29/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.35e-01 | |||||||||||
TGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAT > W3110S.gb/2325851‑2325981 | tGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCg > 1:3262044/1‑71 (MQ=255)tGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCg > 1:2446137/1‑71 (MQ=255)tGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCg > 1:917588/1‑71 (MQ=255)tGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCg > 1:1893632/1‑71 (MQ=255) gCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGc < 1:2126728/71‑1 (MQ=255) gCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGc < 1:623458/71‑1 (MQ=255) aGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTa < 1:2684752/71‑1 (MQ=255) aGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTa < 1:3513678/71‑1 (MQ=255) aGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTa < 1:3659833/71‑1 (MQ=255) aGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTa < 1:173246/71‑1 (MQ=255) gAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTAt < 1:799509/71‑1 (MQ=255) gAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTAt < 1:2875977/71‑1 (MQ=255) gAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTAt < 1:2246838/71‑1 (MQ=255) gAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTAt < 1:1028874/71‑1 (MQ=255) gAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTAt < 1:984120/71‑1 (MQ=255) gCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGt < 1:1775128/69‑1 (MQ=255) gCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGt < 1:2695671/69‑1 (MQ=255) cacaCTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAAc > 1:1496675/1‑54 (MQ=255) cacaCTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTa > 1:3106764/1‑69 (MQ=255) cacaCTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTa > 1:1484123/1‑69 (MQ=255) cacaCTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTa > 1:147130/1‑69 (MQ=255) cacaCTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTa > 1:1424454/1‑69 (MQ=255) cacaCTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTa > 1:1359191/1‑69 (MQ=255) cacaCTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTa > 1:2615109/1‑69 (MQ=255) aacacTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTa > 1:2870264/2‑69 (MQ=255) cTGATGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTgcg < 1:1534133/71‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:3428233/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:572486/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:75565/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:459238/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:3990267/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:3878885/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:3481063/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:3448444/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:3380686/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:2836749/70‑1 (MQ=255) aTGGCTGGCGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGcc < 1:2292663/70‑1 (MQ=255) ctggcGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCcaacaa < 1:2214483/71‑1 (MQ=255) ctggaGTCGCGCATGAAGTACGTTATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCcaacaa < 1:841467/71‑1 (MQ=255) gcGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAAcc < 1:3341337/71‑1 (MQ=255) gcGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAAcc < 1:1653375/71‑1 (MQ=255) gcGTCGCGCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAAcc < 1:2238997/71‑1 (MQ=255) gTCGCGCATGAAGTACGTGATCCGTTAACGGCTAt < 1:1152935/35‑1 (MQ=255) cgcgCATGAAGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGt > 1:2952384/1‑70 (MQ=255) aaGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACaaa > 1:1718751/1‑57 (MQ=255) aaGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACaaa > 1:798421/1‑57 (MQ=255) aaGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACaaa > 1:268338/1‑57 (MQ=255) aaGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACaaa > 1:2867546/1‑57 (MQ=255) aaGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACaaa > 1:28855/1‑57 (MQ=255) aaGTACGTGATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACaaa > 1:1444425/1‑57 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATc > 1:553348/1‑39 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt > 1:3631209/1‑69 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt > 1:579785/1‑69 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt > 1:503949/1‑69 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt > 1:473728/1‑69 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt > 1:3678331/1‑69 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt > 1:2020901/1‑69 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt > 1:2160127/1‑69 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt > 1:2367186/1‑69 (MQ=255) cGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAt > 1:2537553/1‑69 (MQ=255) | TGGCGCAAGCAGAACGCCTCGCCACACTGGGTGAGCTGATGGCTGGCGTCGCGCATGAAGTACGTAATCCGTTAACGGCTATTCGTGGTTATGTACAGATCTTGCGCCAACAAACCAGTGACCCAATACAT > W3110S.gb/2325851‑2325981 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |