Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,350,543	G→A	V337I (GTA→ATA)	nrdA →	ribonucleoside diphosphate reductase 1, alpha subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,350,543	0	G	A	100.0%	271.3 / NA	83	V337I (GTA→ATA)	nrdA	ribonucleoside diphosphate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (29/54);  total (29/54)

GCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTT  >  W3110S.gb/2350478‑2350601                                                                  |                                                           gCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACaa                                                       <  1:101708/71‑1 (MQ=255) gCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACaa                                                       <  1:2463073/71‑1 (MQ=255) gCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACaa                                                       <  1:3558464/71‑1 (MQ=255) gCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACaa                                                       <  1:649379/71‑1 (MQ=255) gCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACaa                                                       <  1:3839654/71‑1 (MQ=255)  ccTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACaa                                                       <  1:3254375/70‑1 (MQ=255)              gAAAAACTACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:3992957/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:3836113/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:2668857/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:3594301/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:1679444/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:3401488/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:3114569/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:1933904/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:206116/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:2700078/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:2346470/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:2685815/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:1575874/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:3838977/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:1481483/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:110991/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:1473831/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:672646/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:1365002/70‑1 (MQ=255)              gAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTg                                           <  1:1360775/70‑1 (MQ=255)               aaaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGa                                          <  1:15345/70‑1 (MQ=255)                aaaaCAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg                                        <  1:2077463/71‑1 (MQ=255)                                   aGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGagtc                     >  1:2750961/1‑68 (MQ=255)                                   aGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGagtc                     >  1:1155202/1‑68 (MQ=255)                                   aGGCAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGagtc                     >  1:2072840/1‑68 (MQ=255)                                   aGGCAACCGCGTGAGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGagtc                     >  1:1657976/1‑68 (MQ=255)                                      cAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGaaa                      >  1:274909/1‑67 (MQ=255)                                      cAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGaaa                      >  1:2314768/1‑67 (MQ=255)                                      cAACCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGaaa                      >  1:3456372/1‑67 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:4077315/71‑1 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:3343186/71‑1 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:697916/71‑1 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:861982/71‑1 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:1019522/71‑1 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:1024359/71‑1 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:1087696/71‑1 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:1567013/71‑1 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:193078/71‑1 (MQ=255)                                        aCCGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGaa                <  1:1716274/71‑1 (MQ=255)                                         ccGGGTGCGTCATATGGACTACGGGATACAAATCCACAAACTGATGTTTACCCGTCTGCTGAAAGGTGaa                <  1:960645/70‑1 (MQ=255)                                         ccGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATg                                        >  1:3687097/1‑46 (MQ=255)                                         ccGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTg                  >  1:3842707/1‑68 (MQ=255)                                         ccGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTg                  >  1:3723176/1‑68 (MQ=255)                                         ccGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTg                  >  1:3548234/1‑68 (MQ=255)                                         ccGCGTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTg                  >  1:3007600/1‑68 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:4032313/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:3441627/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:2299087/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:1916725/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:59864/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:1662212/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:4001412/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:636833/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:695322/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:2559983/71‑1 (MQ=255)                                          cgcgTGCGTCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGa              <  1:1315063/71‑1 (MQ=255)                                                  tCATATGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCAccc      <  1:664022/71‑1 (MQ=255)                                                    atatGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTg    <  1:526173/71‑1 (MQ=255)                                                    atatGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTg    <  1:814152/71‑1 (MQ=255)                                                     tatGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTg    <  1:1672264/70‑1 (MQ=255)                                                      atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCCCCCTGtt  >  1:1909756/1‑71 (MQ=255)                                                      atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:721221/1‑71 (MQ=255)                                                      atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:571642/1‑71 (MQ=255)                                                      atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:342415/1‑71 (MQ=255)                                                      atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:1418748/1‑71 (MQ=255)                                                      atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:1461112/1‑71 (MQ=255)                                                      atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:1933174/1‑71 (MQ=255)                                                      atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGt   >  1:391977/1‑70 (MQ=255)                                                      atGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACACTGtt  >  1:4010915/1‑71 (MQ=255)                                                       tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATc          >  1:736436/1‑62 (MQ=255)                                                       tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:1404365/1‑70 (MQ=255)                                                       tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:625530/1‑70 (MQ=255)                                                       tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:1148018/1‑70 (MQ=255)                                                       tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:1944131/1‑70 (MQ=255)                                                       tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:2067651/1‑70 (MQ=255)                                                       tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:1576435/1‑70 (MQ=255)                                                       tGGACTACGGGATACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGtt  >  1:2359836/1‑70 (MQ=255)                                                               gggaTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTg    <  1:3344943/60‑1 (MQ=255)                                                                  |                                                           GCCTGCTGGTGTTGAAAAACAACCGTGGTGTGGAAGGCAACCGCGTGCGTCATATGGACTACGGGGTACAAATCAACAAACTGATGTATACCCGTCTGCTGAAAGGTGAAGATATCACCCTGTT  >  W3110S.gb/2350478‑2350601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑