Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	259,305	G→A	A7V (GCA→GTA)	phoE ←	outer membrane phosphoporin protein E

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	259,305	0	G	A	100.0%	105.3 / NA	34	A7V (GCA→GTA)	phoE	outer membrane phosphoporin protein E
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (15/19);  total (15/19)

CCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGG  >  W3110S.gb/259239‑259364                                                                   |                                                            ccGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCa                                                          <  1:1903274/70‑1 (MQ=255) ccGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCa                                                          <  1:2045310/70‑1 (MQ=255)             tatatTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCa                                                          <  1:86932/58‑1 (MQ=255)                   tCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCAGAGTGCTCTttttcatttt                                       >  1:3533899/1‑71 (MQ=255)                       cAGCCTTTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTttcattttcatt                                   <  1:2048101/71‑1 (MQ=255)                       cAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTttcattttcatt                                   <  1:4114963/71‑1 (MQ=255)                       cAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTttcattttcatt                                   <  1:3181042/71‑1 (MQ=255)                       cAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTttcattttcatt                                   <  1:3118080/71‑1 (MQ=255)                       cAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTttcattttcatt                                   <  1:3105089/71‑1 (MQ=255)                       cAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTttcattttcatt                                   <  1:2829860/71‑1 (MQ=255)                       cAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTttcattttcatt                                   <  1:2538374/71‑1 (MQ=255)                        aGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTttcattttcatt                                   <  1:1031950/70‑1 (MQ=255)                                 agatgcagatgcCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTtta                         <  1:3545468/71‑1 (MQ=255)                                  gatgcagatgcCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTtta                         <  1:22445/70‑1 (MQ=255)                                        gatgcCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAAcg                  >  1:2888434/1‑71 (MQ=255)                                        gatgcCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAAcg                  >  1:2425051/1‑71 (MQ=255)                                        gatgcCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAAcg                  >  1:1877406/1‑71 (MQ=255)                                          tgcCACAATGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAAcgcg                >  1:3811599/1‑71 (MQ=255)                                                 aTGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAatat         <  1:3504329/71‑1 (MQ=255)                                                 aTGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAatat         <  1:3508996/71‑1 (MQ=255)                                                  tGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGaa            <  1:4123478/67‑1 (MQ=255)                                                  tGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGaa            <  1:178262/67‑1 (MQ=255)                                                  tGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGaa            <  1:1260830/67‑1 (MQ=255)                                                  tGCCCATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGaa            <  1:367909/67‑1 (MQ=255)                                                    cccATCACCACTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAata          >  1:1849925/1‑67 (MQ=255)                                                          accacTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCgg  >  1:1041638/1‑69 (MQ=255)                                                          accacTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCgg  >  1:51094/1‑69 (MQ=255)                                                          accacTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCgg  >  1:116626/1‑69 (MQ=255)                                                          accacTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCgg  >  1:3837109/1‑69 (MQ=255)                                                          accacTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCgg  >  1:3645087/1‑69 (MQ=255)                                                          accacTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCgg  >  1:3623016/1‑69 (MQ=255)                                                          accacTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCgg  >  1:1852826/1‑69 (MQ=255)                                                          accacTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCgg  >  1:2223325/1‑69 (MQ=255)                                                          accacTAATACCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCgg  >  1:228552/1‑69 (MQ=255)                                                                   |                                                            CCGTCTTTATTATATATTTCTGCAGCCTGTACAGATGCAGATGCCACAATGCCCATCACCACTAATGCCAGAGTGCTCTTTTTCATTTTCATTCCTGATTTTAATTAACGCGCGAATATTCAGCGG  >  W3110S.gb/259239‑259364

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑