Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,397,191	A→G	F174S (TTC→TCC)	nuoM ←	NADH:ubiquinone oxidoreductase, membrane subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,397,191	0	A	G	100.0%	221.0 / NA	63	F174S (TTC→TCC)	nuoM	NADH:ubiquinone oxidoreductase, membrane subunit M
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (50/13);  total (50/13)

TGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGT  >  W3110S.gb/2397125‑2397247                                                                   |                                                         tGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACtt                                                      <  1:500138/71‑1 (MQ=255)  gAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg                                                     >  1:1780584/1‑71 (MQ=255)  gAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg                                                     >  1:3308369/1‑71 (MQ=255)  gAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg                                                     >  1:3325590/1‑71 (MQ=255)  gAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg                                                     >  1:3452423/1‑71 (MQ=255)  gAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg                                                     >  1:3123477/1‑71 (MQ=255)  gAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg                                                     >  1:3821829/1‑71 (MQ=255)  gAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg                                                     >  1:1581482/1‑71 (MQ=255)  gAACAAAAACCAGCGCCAGGATGGCAATCAACATCACAAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg                                                     >  1:3427992/1‑71 (MQ=255)  gAACAAAAACCAGCGCCAGGATGGCAATCAACATAACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTg                                                     >  1:738109/1‑71 (MQ=255)   aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg                                                    >  1:1071880/1‑71 (MQ=255)   aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg                                                    >  1:2796916/1‑71 (MQ=255)   aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg                                                    >  1:2526237/1‑71 (MQ=255)   aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg                                                    >  1:3383303/1‑71 (MQ=255)   aaCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg                                                    >  1:1299663/1‑71 (MQ=255)   aaCAAAAACCAGCGCCAGGATGGAAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTgg                                                    >  1:3707637/1‑71 (MQ=255)                       tGGCAATTAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtt                                >  1:1947347/1‑71 (MQ=255)                       tGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtt                                >  1:2398990/1‑71 (MQ=255)                         gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtttt                              <  1:4132554/71‑1 (MQ=255)                         gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGtttt                              <  1:450833/71‑1 (MQ=255)                         gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGttt                               >  1:180846/1‑70 (MQ=255)                         gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGttt                               >  1:3442919/1‑70 (MQ=255)                         gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGttt                               >  1:3850055/1‑70 (MQ=255)                         gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGttt                               >  1:942531/1‑70 (MQ=255)                         gCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGttt                               >  1:3071601/1‑70 (MQ=255)                            aTCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGt                                          <  1:1518136/56‑1 (MQ=255)                             tCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCg                          <  1:1595867/71‑1 (MQ=255)                               aaCATCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGt                         >  1:1092778/1‑70 (MQ=255)                                   tCACCAGGCCACTCGCGTGGGTGTAAATGAAGGACtt                                                      >  1:3018641/1‑37 (MQ=38)                                   tCACCAGGCCACTCGCCTGGGTGTAAATGGAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                    >  1:3519755/1‑71 (MQ=255)                                   tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                    >  1:3152443/1‑71 (MQ=255)                                   tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                    >  1:439495/1‑71 (MQ=255)                                   tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                    >  1:3002542/1‑71 (MQ=255)                                   tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                    >  1:1215211/1‑71 (MQ=255)                                   tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                    >  1:1562288/1‑71 (MQ=255)                                   tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                    >  1:1562505/1‑71 (MQ=255)                                   tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                    >  1:1879992/1‑71 (MQ=255)                                   tCACCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCaga                     >  1:994852/1‑70 (MQ=255)                                   tCACCAGGCCACTCGCCTGGCTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCagag                    >  1:2878666/1‑71 (MQ=255)                                     aCCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATAcgc                                   >  1:2918983/1‑54 (MQ=255)                                     aCCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc                  >  1:2312961/1‑71 (MQ=255)                                     aCCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc                  >  1:3189007/1‑71 (MQ=255)                                     aCCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc                  >  1:1458373/1‑71 (MQ=255)                                     aCCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc                  >  1:3866444/1‑71 (MQ=255)                                     aCCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc                  >  1:1822310/1‑71 (MQ=255)                                     aCCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc                  >  1:1847385/1‑71 (MQ=255)                                     aCCAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGc                  >  1:2511274/1‑71 (MQ=255)                                       cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTgc                                                >  1:4030392/1‑39 (MQ=255)                                       cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATa                                      >  1:1600528/1‑49 (MQ=255)                                       cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATAcg                                    >  1:3629239/1‑51 (MQ=255)                                       cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATAcg                                    >  1:2831969/1‑51 (MQ=255)                                       cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATAcg                                    >  1:1612661/1‑51 (MQ=255)                                       cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATAcg                                    >  1:422099/1‑51 (MQ=255)                                       cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt                <  1:1713028/71‑1 (MQ=255)                                       cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt                <  1:588444/71‑1 (MQ=255)                                       cAGGCCACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCtt                <  1:113528/71‑1 (MQ=255)                                           ccACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa              >  1:2054552/1‑69 (MQ=255)                                           ccACTCGCCTGGGTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTa              >  1:1905614/1‑69 (MQ=255)                                                      ggTGTAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAg   <  1:3936353/69‑1 (MQ=255)                                                        tgtAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGt  <  1:2830588/68‑1 (MQ=255)                                                        tgtAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGt  <  1:535864/68‑1 (MQ=255)                                                        tgtAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGt  <  1:3706570/68‑1 (MQ=255)                                                        tgtAAATGAAGGACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGt  <  1:3435677/68‑1 (MQ=255)                                                                   |                                                         TGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGT  >  W3110S.gb/2397125‑2397247

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑