Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,427,890	T→G	I246S (ATT→AGT)	yfcH →	conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,427,890	0	T	G	100.0%	83.6 / NA	24	I246S (ATT→AGT)	yfcH	conserved hypothetical protein with NAD(P)‑binding Rossmann‑fold domain
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/16);  total (8/16)

TACGCAATGAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCATTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAA  >  W3110S.gb/2427836‑2427933                                                       |                                            tACGCAATGAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCcgcg                             <  1:3388397/71‑1 (MQ=255)        tGAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCa                      <  1:1574008/71‑1 (MQ=255)                   cccATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt            <  1:3981675/70‑1 (MQ=255)                   cccATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt            <  1:1528920/70‑1 (MQ=255)                   cccATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt            <  1:3454775/70‑1 (MQ=255)                   cccATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt            <  1:3135330/70‑1 (MQ=255)                   cccATGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt            <  1:3132208/70‑1 (MQ=255)                       tGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt            <  1:1348421/66‑1 (MQ=255)                       tGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt            <  1:1622682/66‑1 (MQ=255)                       tGCGCTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt            <  1:329891/66‑1 (MQ=255)                         cgcTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGc                        >  1:3469691/1‑52 (MQ=255)                         cgcTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTaa          >  1:1371161/1‑66 (MQ=255)                         cgcTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTaa          >  1:1805328/1‑66 (MQ=255)                         cgcTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGc     >  1:3106797/1‑71 (MQ=255)                         cgcTCGGTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCAATTCGACTGTTAATGGGc     >  1:2490262/1‑71 (MQ=255)                              ggTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGaa  <  1:3209075/69‑1 (MQ=255)                              ggTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGaa  <  1:2089822/69‑1 (MQ=255)                              ggTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGaa  <  1:2054745/69‑1 (MQ=255)                              ggTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGaa  <  1:562266/69‑1 (MQ=255)                              ggTCATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCACCGCGACCGCCATTCGACTGTTAATGGGCGaa  <  1:1431513/69‑1 (MQ=255)                                 cATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCAtt                    >  1:453190/1‑48 (MQ=255)                                 cATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTc                   >  1:2625831/1‑49 (MQ=255)                                 cATGCGCTGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTc                   >  1:3771185/1‑49 (MQ=255)                                        tGCATCGCCCGGCCAGTTTGCGCGTCCCCGCGACCGCCATTCGACTGtt            <  1:1151007/49‑1 (MQ=255)                                                       |                                            TACGCAATGAACAATTTGCCCATGCGCTCGGTCATGCGCTGCATCGCCCGGCCATTTTGCGCGTCCCCGCGACCGCCATTCGACTGTTAATGGGCGAA  >  W3110S.gb/2427836‑2427933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑