Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,453,614	A→G	A91A (GCT→GCC)	prmB ←	N5‑glutamine methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,453,614	0	A	G	100.0%	102.9 / NA	30	A91A (GCT→GCC)	prmB	N5‑glutamine methyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/14);  total (16/14)

CGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGG  >  W3110S.gb/2453548‑2453678                                                                   |                                                                 cgGCACCAGCACGCGTTCATCGACGTAAAATTCATGGGCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCa                                                              <  1:3174719/71‑1 (MQ=255) cgGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCa                                                              <  1:107664/71‑1 (MQ=255) cgGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCa                                                              <  1:2101183/71‑1 (MQ=255) cgGCACCAGCAAGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCa                                                              <  1:1807680/71‑1 (MQ=255)                  cATCGAGGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCa                                                >  1:1092861/1‑68 (MQ=255)                  cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGtt                                                  >  1:230204/1‑66 (MQ=255)                  cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGtt                                                  >  1:1405766/1‑66 (MQ=255)                  cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCa                                                >  1:867703/1‑68 (MQ=255)                  cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCa                                                >  1:2135214/1‑68 (MQ=255)                  cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCa                                                >  1:2024760/1‑68 (MQ=255)                  cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCa                                                >  1:361088/1‑68 (MQ=255)                  cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCa                                                >  1:3972937/1‑68 (MQ=255)                  cATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCa                                                >  1:1544867/1‑68 (MQ=255)                              aTTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGAt                                  >  1:1675406/1‑70 (MQ=255)                              aTTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGAt                                  >  1:2226043/1‑70 (MQ=255)                              aTTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATT‑ACGCGGCGGATCa                                >  1:1004969/1‑71 (MQ=255)                               ttCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGAGGcggcgg                                    <  1:2066552/67‑1 (MQ=255)                                         cAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTcaac                      <  1:3216299/71‑1 (MQ=255)                                         cAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTcaac                      <  1:559467/71‑1 (MQ=255)                                         cAGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTcaac                      <  1:1385492/71‑1 (MQ=255)                                          aGAACCACGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTcaac                      <  1:197203/70‑1 (MQ=255)                                                aCGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACgg               >  1:242255/1‑71 (MQ=255)                                                aCGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACgg               >  1:3407136/1‑71 (MQ=255)                                                aCGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACgg               >  1:1513521/1‑71 (MQ=255)                                                aCGCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACgg               >  1:886230/1‑71 (MQ=255)                                                  gCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCTACAATACGgtg             <  1:1940896/71‑1 (MQ=255)                                                  gCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGgtg             <  1:2042821/71‑1 (MQ=255)                                                  gCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGgtg             <  1:1282624/71‑1 (MQ=255)                                                  gCTTTGTTGGTCAGGTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGgtg             <  1:919732/71‑1 (MQ=255)                                                              acgTAGGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTgg  <  1:3541629/68‑1 (MQ=255)                                                                   |                                                                 CGGCACCAGCACGCGTTCATCGACGTAAAATTCATGGCCGCAGAACCACGCTTTGTTGGTCAGGTAAGCCACCGGAATGCGTTCATTGACGCGGCGGATCACGCGTTCAACAATACGGTGTTTTTCGCTGG  >  W3110S.gb/2453548‑2453678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑