Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,501,495	G→A	S135S (TCG→TCA)	ddg →	palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,501,495	0	G	A	100.0%	81.6 / NA	27	S135S (TCG→TCA)	ddg	palmitoleoyl‑acyl carrier protein (ACP)‑dependent acyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/16);  total (11/16)

TTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCT  >  W3110S.gb/2501427‑2501547                                                                     |                                                     ttGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACt                                                    >  1:532218/1‑71 (MQ=255) ttGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACt                                                    >  1:438869/1‑71 (MQ=255) ttGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACt                                                    >  1:3375578/1‑71 (MQ=255)    gATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTgg                                                  <  1:1995710/70‑1 (MQ=255)      tagccTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAAc                                               >  1:1771073/4‑71 (MQ=255)      tAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAAc                                               >  1:741975/1‑71 (MQ=255)      tAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAAc                                               >  1:2919494/1‑71 (MQ=255)        aCCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTg                                             <  1:242690/71‑1 (MQ=255)        aCCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTg                                             <  1:885324/71‑1 (MQ=255)        aCCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTg                                             <  1:3003773/71‑1 (MQ=255)        aCCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTg                                             <  1:1800444/71‑1 (MQ=255)        aCCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTg                                             <  1:2235664/71‑1 (MQ=255)            tAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGggcg                                         >  1:3671175/1‑71 (MQ=255)            tAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGggcg                                         >  1:2439438/1‑71 (MQ=255)              aaCGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGggcggc                                       <  1:401714/71‑1 (MQ=255)              aaCGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGggcggc                                       <  1:2588721/71‑1 (MQ=255)              aaCGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGggcggc                                       <  1:770920/71‑1 (MQ=255)                                aTCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTgg                                                  <  1:3768308/42‑1 (MQ=255)                                aTCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGaa                                                >  1:959198/1‑44 (MQ=255)                                aTCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGc                     >  1:172251/1‑71 (MQ=255)                                        gTAATGGTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAat             <  1:3084059/71‑1 (MQ=255)                                              gTTGTCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGc       >  1:343032/1‑71 (MQ=255)                                                  tCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGccaacc                <  1:3494773/58‑1 (MQ=255)                                                  tCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGccaacc                <  1:2317449/58‑1 (MQ=255)                                                  tCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGccaacc                <  1:929877/58‑1 (MQ=255)                                                  tCGGCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGccaacc                <  1:966649/58‑1 (MQ=255)                                                    ggCGTCCATTTTATGTCACTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCt  <  1:3300962/70‑1 (MQ=255)                                                                     |                                                     TTGGATAACCTTAAACGCGCACAAATGCAAAATCGCGGCGTAATGGTTGTCGGCGTCCATTTTATGTCGCTGGAACTGGGCGGCCGCGTGATGGGACTGTGCCAACCAATGATGGCTACCT  >  W3110S.gb/2501427‑2501547

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑