| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,541,664 | G→A | E129K (GAA→AAA) | crr → | glucose‑specific enzyme IIA component of PTS |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,541,664 | 0 | G | A | 88.0% | 137.6 / 10.2 | 50 | E129K (GAA→AAA) | crr | glucose‑specific enzyme IIA component of PTS |
| Reads supporting (aligned to +/- strand): ref base G (2/4); new base A (16/28); total (18/32) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAA > W3110S.gb/2541599‑2541717 | ttGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGaaa > 1:719067/1‑68 (MQ=255)ttGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGaaa > 1:1388689/1‑68 (MQ=255)ttGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGaaa > 1:1543102/1‑68 (MQ=255) gCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAgaga > 1:62558/1‑70 (MQ=255) gCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAgaga > 1:860196/1‑70 (MQ=255) gCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAagag > 1:375708/1‑69 (MQ=255) gCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAgaga > 1:3939233/1‑70 (MQ=255) gCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAgaga > 1:1340861/1‑70 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAag < 1:2609665/61‑1 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAag < 1:176497/61‑1 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAag < 1:2469160/61‑1 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAag < 1:503040/61‑1 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAag < 1:3644875/61‑1 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGcc > 1:1556571/1‑69 (MQ=255) gaagGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGcc > 1:2296677/1‑69 (MQ=255) tCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAag < 1:3959138/56‑1 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTAc < 1:2755145/70‑1 (MQ=255) aGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTAc < 1:526470/70‑1 (MQ=255) gcgcGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTAc < 1:546298/69‑1 (MQ=255) cgcgTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAgaga > 1:2062489/1‑55 (MQ=255) gTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGAc < 1:1932940/71‑1 (MQ=255) gTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGAc < 1:1371711/71‑1 (MQ=255) tGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGAc < 1:1888073/70‑1 (MQ=255) aaaGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGGCTcc > 1:3719728/1‑71 (MQ=255) aaaGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTcc > 1:2367289/1‑71 (MQ=255) aaaGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTcc > 1:662072/1‑71 (MQ=255) aaaGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTcc > 1:2871158/1‑71 (MQ=255) aaaGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTcc > 1:1067945/1‑71 (MQ=255) aaaGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTc > 1:549120/1‑70 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt < 1:1254103/71‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt < 1:685545/71‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt < 1:3201690/71‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgtt < 1:2186491/71‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt < 1:1685050/70‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt < 1:184280/70‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt < 1:1951778/70‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt < 1:1717640/70‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt < 1:734174/70‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt < 1:2601666/70‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt < 1:3920907/70‑1 (MQ=255) gCGATACTGTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGgttgt < 1:3912478/70‑1 (MQ=255) gTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCaa > 1:3900159/1‑71 (MQ=255) gTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCaa > 1:2237847/1‑71 (MQ=255) gTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCaa > 1:2731131/1‑71 (MQ=255) gTCATTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCaa > 1:2823050/1‑71 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTa < 1:2667570/43‑1 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTa < 1:2297323/43‑1 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTa < 1:3612448/43‑1 (MQ=255) aTTGAATTTGATCTGCCGCTGCTGAAAGAGAAAGCCAAGTCTa < 1:1764586/43‑1 (MQ=255) tGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGaa < 1:231135/70‑1 (MQ=255) tGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGaa < 1:3066065/70‑1 (MQ=255) tGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGaa < 1:3228517/70‑1 (MQ=255) gATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGaa < 1:2110507/69‑1 (MQ=255) | TTGCTGAAGAAGGTCAGCGCGTGAAAGTTGGCGATACTGTCATTGAATTTGATCTGCCGCTGCTGGAAGAGAAAGCCAAGTCTACCCTGACTCCGGTTGTTATCTCCAACATGGACGAA > W3110S.gb/2541599‑2541717 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |