| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | W3110S.gb | 2,589,664 | A→T | intergenic (+300/‑239) | acrD → / → yffB | aminoglycoside/multidrug efflux system/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | W3110S.gb | 2,589,664 | 0 | A | T | 93.3% | 249.9 / 7.1 | 89 | intergenic (+300/‑239) | acrD/yffB | aminoglycoside/multidrug efflux system/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base T (18/65); total (23/66) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAA > W3110S.gb/2589602‑2589723 | aCTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAg > 1:1074056/1‑70 (MQ=255)aCTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAg > 1:2889731/1‑70 (MQ=255)aCTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAg > 1:3547726/1‑70 (MQ=255) ccTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa < 1:2028614/69‑1 (MQ=255) ccTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACa < 1:2096951/71‑1 (MQ=255) ccTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACa < 1:2101592/71‑1 (MQ=255) ccTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACa < 1:2678581/71‑1 (MQ=255) cGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACAtt < 1:875089/69‑1 (MQ=255) cGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACAtt < 1:1784917/69‑1 (MQ=255) cGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACAtt < 1:2013498/69‑1 (MQ=255) cGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACAtt < 1:3971296/69‑1 (MQ=255) cGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACAtt < 1:2361260/69‑1 (MQ=255) ggggACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCga < 1:812764/71‑1 (MQ=255) gggACAAATCTTATGTAAATACTATTGTCCTGCGGTGATGATTTGTATGTGTTACACAGCaa < 1:1832177/62‑1 (MQ=255) gggACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCgag < 1:1140426/71‑1 (MQ=255) gggACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCgag < 1:2145034/71‑1 (MQ=255) gggACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCgag < 1:1174221/71‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:2641511/70‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:3730581/70‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:2142617/70‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:3899074/70‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:429560/70‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:472819/70‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:557413/70‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:1464272/70‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:1676093/70‑1 (MQ=255) tAAATACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:1539797/70‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCt < 1:1725117/71‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCt < 1:2740124/71‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCt < 1:1436743/71‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCt < 1:1023495/71‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCt < 1:3008810/71‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCt < 1:2453334/71‑1 (MQ=255) aaTACTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCt < 1:4057757/71‑1 (MQ=255) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAAt > 1:2318393/1‑70 (MQ=255) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAAt > 1:788835/1‑70 (MQ=255) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATa > 1:4020187/1‑71 (MQ=255) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATa > 1:3861197/1‑71 (MQ=255) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATa > 1:3798896/1‑71 (MQ=255) cTATGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATa > 1:2124974/1‑71 (MQ=255) tGGTCCTGCGGTGATGATTTGTATTTGTTACACAGCAACATTTCGAGATAtt < 1:2031483/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGTAACATTTCGAGATAtt < 1:1395581/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:1263565/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:516799/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:3988014/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:1161024/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:993416/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:1908240/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:2644037/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:2269302/52‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:2885260/54‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:2951925/54‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:2521457/54‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:164602/54‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:3308624/54‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:1548108/54‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:2166457/54‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:1341126/54‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:148903/54‑1 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATc > 1:1144179/1‑63 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATc > 1:728614/1‑63 (MQ=255) tGGTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATc > 1:3859182/1‑63 (MQ=255) ggTCCTGCGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:2660148/53‑1 (MQ=255) tCCTGCGGTGATGATTTGTATGTGTTACACAGCAACa < 1:3670704/37‑1 (MQ=255) cTGCGGTGAGGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCa < 1:1353582/49‑1 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGGGATATTCATACGGCATCTAATACTGATTTAAtt > 1:3975865/1‑71 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTaa < 1:1423658/69‑1 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTaa < 1:1228443/69‑1 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTaa < 1:2152268/69‑1 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTaa < 1:3480684/69‑1 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTaa < 1:922795/69‑1 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTaa < 1:3539850/69‑1 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAAtt > 1:3354966/1‑71 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAAtt > 1:3320461/1‑71 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAAt > 1:2317019/1‑70 (MQ=255) cGGTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATACTCATACGGCATCTAATACTGATTTAAt > 1:3197095/1‑70 (MQ=255) ggTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTaa < 1:313704/68‑1 (MQ=255) gTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:1718435/42‑1 (MQ=255) gTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:12338/42‑1 (MQ=255) gTGATGATTTGTATGTGTTACACAGCAACATTTCGAGATAtt < 1:581127/42‑1 (MQ=255) gTGATGATTTGTATGTGTTACACAGCAACATATCGAGATAtt < 1:1723391/42‑1 (MQ=38) gatTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGtt > 1:3323035/1‑70 (MQ=255) gatTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGtt > 1:2267172/1‑70 (MQ=255) gatTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGtt > 1:3379086/1‑70 (MQ=255) gatTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGtt > 1:2712701/1‑70 (MQ=255) gatTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGtt > 1:3599937/1‑70 (MQ=255) atTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTaa < 1:956846/71‑1 (MQ=255) atTTGTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTa > 1:576413/1‑70 (MQ=255) gTATGTGTTACACAGCAACATTTCGAGATATTCATACGGCa < 1:2360919/41‑1 (MQ=255) | ACTACCTCCGGGGGACAAATCTTATGTAAATACTATGGTCCTGCGGTGATGATTTGTATGTGATACACAGCAACATTTCGAGATATTCATACGGCATCTAATACTGATTTAATTCTGGTTAA > W3110S.gb/2589602‑2589723 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |