Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,697,585	(A)5→4	coding (171/849 nt)	yfhH →	predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,697,581	0	A	.	100.0%	161.6 / NA	37	coding (167/849 nt)	yfhH	predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (37/0);  total (37/0)

GAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCG  >  W3110S.gb/2697541‑2697606                                         |                          gaggCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1630910/4‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaaact                      >  1:756104/1‑44 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCaaa                         >  1:446631/1‑43 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCtat              >  1:1404349/1‑53 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCta               >  1:1786276/1‑52 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTcc   >  1:945415/1‑64 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTcc   >  1:1263972/1‑64 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTcc   >  1:435552/1‑64 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTcc   >  1:254606/1‑64 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTcc   >  1:1452706/1‑64 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTcc   >  1:1717954/1‑64 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTcc   >  1:605294/1‑64 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:676507/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:412996/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:420054/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:44733/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:646457/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:976264/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:697044/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:812170/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:875911/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:921488/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1028534/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:373788/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:286830/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1857023/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1733430/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1626459/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1513006/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1504866/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1497725/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:132174/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1311575/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1266502/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1255286/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:1250116/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCCCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:255308/1‑65 (MQ=255) gAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTAGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:671802/1‑65 (MQ=255) gAAGCCGGAGT‑AGTCAGTCCAGCGTCGTGAAGTTCGCGC‑AAAACTCGGCTATAAAGGTTTTCCg  >  1:189833/1‑64 (MQ=255)                                         |                          GAAGCCGGAGTCAGTCAGTCCAGCGTCGTGAAGTTCGCGCAAAAACTCGGCTATAAAGGTTTTCCG  >  W3110S.gb/2697541‑2697606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑