Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,439,396	(G)5→4	coding (383/774 nt)	nudC ←	NADH pyrophosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,439,392	0	G	.	100.0%	366.1 / NA	82	coding (387/774 nt)	nudC	NADH pyrophosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (0/82);  total (0/82)

TGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCAGGGGGCGATTTGCGGG  >  W3110S.gb/3439342‑3439407                                                   |                tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑TGGGCGATTTGCggg  <  1:1164971/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGTggg  <  1:1419121/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:266695/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:385126/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:363794/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:333633/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:32894/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:324020/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:319859/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:316399/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:312285/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:282207/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:410500/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1874457/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1829449/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1821933/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1806058/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:180278/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1801678/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1042336/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1797116/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:764941/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:98424/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:977931/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:967336/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:928863/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:906853/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:895347/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:822601/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:7929/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:784213/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:4054/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:732806/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:577801/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:554053/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:543167/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:540785/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:499039/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:473704/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1801527/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1309022/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:156098/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1791543/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1526747/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1517996/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1511850/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1508000/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1408472/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1373208/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1547765/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1265181/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1254462/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1202938/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1161796/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1140416/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1123559/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1073227/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1066911/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:168383/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1790567/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1789596/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1777466/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1751388/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:175113/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1695296/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1591672/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1661051/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1648890/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1627340/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1626734/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:162442/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1623318/65‑1 (MQ=255) tGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1621161/65‑1 (MQ=255) tGCTGGGCGAGGAGGAGCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:442627/65‑1 (MQ=255) tGCGGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:122838/65‑1 (MQ=255)  gCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:139614/64‑1 (MQ=255)  gCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:65346/64‑1 (MQ=255)  gCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:240199/64‑1 (MQ=255)  gCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:845628/64‑1 (MQ=255)  gCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1575629/64‑1 (MQ=255)    tGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:199142/62‑1 (MQ=255)                   gAATCATCGCGACGGATGGCAACAATAATGCA‑GGGGCGATTTGCggg  <  1:1781420/47‑1 (MQ=255)                                                   |                TGCTGGGCGAGGAGGATCGAATCATCGCGACGGATGGCAACAATAATGCAGGGGGCGATTTGCGGG  >  W3110S.gb/3439342‑3439407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑