Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	3,811,498	Δ1 bp	intergenic (‑26/‑254)	yicE ← / → gltS	predicted transporter/glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	3,811,498	0	C	.	100.0%	165.9 / NA	38	intergenic (‑26/‑254)	yicE/gltS	predicted transporter/glutamate transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base . (38/0);  total (38/0)

CAGACATCGAAAACAATCCCGTGGTAGCAAAGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAA  >  W3110S.gb/3811466‑3811531                                 |                                  cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:729551/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:383953/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:417426/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:485465/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:494446/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:544070/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:617575/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:633922/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:675445/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:728994/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:379623/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:830761/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:896132/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:899350/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:914132/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:92452/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:93391/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:94797/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:969901/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1721560/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1064140/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1116946/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1178239/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1232566/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1249807/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1299272/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1378407/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1468415/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1630920/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1045453/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1769565/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1856506/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1864858/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1871750/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:1877980/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:216442/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:339876/1‑65 (MQ=255) cAGACATCGAAAACAATCCCGTGGTAGCAAAG‑GGGCATTTTAGCTGACCGAAAGAGAAAAGCaaa  >  1:356681/1‑65 (MQ=255)                                 |                                  CAGACATCGAAAACAATCCCGTGGTAGCAAAGCGGGCATTTTAGCTGACCGAAAGAGAAAAGCAAA  >  W3110S.gb/3811466‑3811531

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑