Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,574,945	+T	coding (1972/2796 nt)	pqqL ←	predicted peptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,574,942	1	.	T	95.5%	71.0 / ‑2.7	22	F659I (TTC→ATC)	pqqL	predicted peptidase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base T (21/0);  minor base G (1/0);  total (22/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

CGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAA‑TTTCTCCGCTGGACGCTGG  >  W3110S.gb/1574898‑1574961                                              |                    cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCt    >  1:1640709/1‑63 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:335740/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:851767/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:808808/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:696506/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:639242/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:616689/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:602828/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:541016/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:453447/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:446440/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:320005/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:1822573/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:1757231/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:1688546/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:1685386/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:1554906/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:1541506/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTgg  >  1:107919/1‑65 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTg   >  1:305862/1‑64 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAATTTTCTCCGCTGGACGCTg   >  1:533306/1‑64 (MQ=255) cGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAAGTTTCTCCGCTGGACGCTgg  >  1:1065195/1‑65 (MQ=255)                                              |                    CGTGCGGTCATCAGCATAGCGCGTCTCATACATCTGCTGGGCGAA‑TTTCTCCGCTGGACGCTGG  >  W3110S.gb/1574898‑1574961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑