Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,707,272	+A	coding (309/441 nt)	ydgK →	conserved inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,707,268	1	.	A	100.0%	115.7 / NA	36	V102E (GTA→GAA)	ydgK	conserved inner membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (36/0);  total (36/0)

CTGGCTGCTTATTTCCGTATTGCTGGCAGT‑AAAAGCATTCGCTTTTTCACCCGTGGAAGATGG  >  W3110S.gb/1707239‑1707301                               |                                  cTGGCTGCTTTTTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:940655/1‑64 (MQ=39) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCtt                     >  1:1736682/1‑45 (MQ=38) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTc                 >  1:272957/1‑49 (MQ=38) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCCCCCGTGGAAGATgg  >  1:1397905/1‑64 (MQ=39) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGATGATgg  >  1:54099/1‑64 (MQ=39) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:76207/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:64155/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:565002/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:842303/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:544722/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:321173/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:488000/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:847531/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:432157/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:414575/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:398369/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:93946/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:360468/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:353512/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:351455/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:343211/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1204633/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:301059/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1843713/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1788130/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1751136/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:169136/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1684368/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1407645/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1392045/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1388825/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1306079/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1277726/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1268031/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATgg  >  1:1222758/1‑64 (MQ=255) cTGGCTGCTTATTTCCGTATTGCTGGCAGTAAAAAGCATTCGCTTTTTCACCCGTGGAAGATg   >  1:1328112/1‑63 (MQ=255)                               |                                  CTGGCTGCTTATTTCCGTATTGCTGGCAGT‑AAAAGCATTCGCTTTTTCACCCGTGGAAGATGG  >  W3110S.gb/1707239‑1707301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑