Predicted mutation
evidence seq id position mutation annotation gene description
RA W3110S.gb 56,155 +C coding (955/2355 nt) imp ← exported protein required for envelope biosynthesis and integrity

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*W3110S.gb56,1511.C94.5% 395.2 / 14.1 110coding (959/2355 nt)impexported protein required for envelope biosynthesis and integrity
Reads supporting (aligned to +/- strand):  ref base . (0/6);  new base C (0/104);  total (0/110)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 110 total aligned reads displayed.

TGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGA‑CCCCGGAGTGGTTCCAGTAGAATAACCAACGA  >  W3110S.gb/56107‑56183
                                             |                                
tGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGA‑CCCCGGAGTGGTTCCAGTAg              <  1:1434244/65‑1 (MQ=255)
tGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGA‑CCCCGGAGTGGTTCCAGTAg              <  1:1601416/65‑1 (MQ=255)
tGACCTTGGTGTAGTCGACGGTGAAACGCCACACCTGATCCATGA‑CCCCGGAGTGGGTCCAGTAg              <  1:1690963/65‑1 (MQ=255)
 gACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGA‑CCCCGGAGTGGTTCCAGTAg              <  1:1169685/64‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGTTTCCAGTAGAATAACCAacga  <  1:446453/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAatga  <  1:690572/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:326881/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:438572/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:437848/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:413198/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:389428/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:379660/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:365022/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:354451/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:342287/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:53057/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:318589/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:314057/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:27752/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:264893/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:254176/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:250863/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1015628/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:212138/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:189721/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:701396/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:9621/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:879333/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:874929/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:871718/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:871161/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:870572/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:811203/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:752870/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:705031/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:703530/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:473427/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:693667/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:663113/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:660985/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:636217/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:633272/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:596844/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:595680/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:593541/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:566241/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:246296/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1183895/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1439355/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1390232/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1379811/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1293675/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1286323/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1250277/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1246727/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1234155/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1219445/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1186076/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1829615/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1144694/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1125828/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1123235/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1116562/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1099932/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1090463/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1089491/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1024934/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1015696/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1647762/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1886632/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1828995/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:178763/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1747864/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1654555/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1467787/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1616627/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1616313/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1590103/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1562786/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:151143/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1509100/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1516133/65‑1 (MQ=255)
             gTCGACGTTGAAACGCCACACCTGATCCATGA‑GCCCGGAGTGGTTCCAGTAg              <  1:1799767/52‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:766980/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1498856/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:230595/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1384214/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:256124/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:855186/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:135825/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:705380/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:29699/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1161152/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:381100/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1217024/64‑1 (MQ=255)
              tCGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:609267/64‑1 (MQ=255)
               cGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:602109/63‑1 (MQ=255)
               cGACGTTGAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:91444/63‑1 (MQ=255)
                   gTTGTAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:1329709/59‑1 (MQ=255)
                      gAAACGCCACACCTGATCCATGACCCCCGGAGTGGTTCCAGTAGAATAACCAacga  <  1:799160/56‑1 (MQ=255)
                                             |                                
TGACCTTGGTGTAGTCGACGTTGAAACGCCACACCTGATCCATGA‑CCCCGGAGTGGTTCCAGTAGAATAACCAACGA  >  W3110S.gb/56107‑56183

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑