Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	1,882,549	+A	intergenic (‑76/+51)	yeaS ← / ← yeaT	neutral amino‑acid efflux system/predicted DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	1,882,546	1	.	A	100.0%	125.5 / NA	34	intergenic (‑73/+54)	yeaS/yeaT	neutral amino‑acid efflux system/predicted DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base A (0/34);  total (0/34)

ATCTGTAGCGGCTTAG‑AAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATC  >  W3110S.gb/1882531‑1882594                 |                                                 aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:649167/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1075675/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:408052/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:434450/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:4743/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:545985/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:595280/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:622875/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:633716/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:386044/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:6540/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:705902/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:728650/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:821918/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:928849/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:940907/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:975832/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1776921/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1133424/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1221563/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1259442/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:141835/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1461418/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1486495/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1541195/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1636684/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:318453/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:178143/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1830230/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1872624/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:1882537/65‑1 (MQ=255) aTCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:25232/65‑1 (MQ=255)  tCTGTAGCGGCTTAGAAAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:56227/64‑1 (MQ=255)         cGGCTTAGAAAACGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATc  <  1:273785/57‑1 (MQ=255)                 |                                                 ATCTGTAGCGGCTTAG‑AAAGGAGAAAATCAGGTTTTAACCTGATATCAACCCGATAATTGAATC  >  W3110S.gb/1882531‑1882594

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑