Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,254,800	+T	coding (627/858 nt)	nfo →	endonuclease IV with intrinsic 3'‑5' exonuclease activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,254,797	1	.	T	98.1%	194.7 / ‑3.2	53	coding (624/858 nt)	nfo	endonuclease IV with intrinsic 3'‑5' exonuclease activity
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base T (52/0);  total (53/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

GTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGC‑T‑TTAAGTATCT  >  W3110S.gb/2254745‑2254808                                                      |             gTACTCCAGCCGAATGCGAGAAAACATTCGCGTATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:47213/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATc   >  1:1779963/1‑64 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATc   >  1:1336863/1‑64 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATc   >  1:1528608/1‑64 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:520968/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1856789/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1862275/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:190172/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:274288/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:275889/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:354107/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:357990/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:458555/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:461480/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:485178/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:491816/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1833942/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:532025/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:606807/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:614507/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:652450/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:654112/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:656157/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:757500/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:762644/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:819242/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:967300/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:974066/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1634876/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1056443/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1059044/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1060098/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1061441/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1082514/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1127683/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1238409/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1275490/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1331890/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1520907/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1556084/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:184916/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1654617/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1664932/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1680538/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1685944/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1697724/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1731743/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1769/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:177119/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1793573/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1029394/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGCTT‑TTAAGTATCt  >  1:1842360/1‑65 (MQ=255) gTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGC‑TATTAAGTATCt  >  1:1509130/1‑65 (MQ=255)                                                      |             GTACTCCAGCCGAATGCGAGAAAACATTCGCGGATTTTGCCCGTACTGTCGGC‑T‑TTAAGTATCT  >  W3110S.gb/2254745‑2254808

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑