Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	2,462,875	(T)6→7	coding (1731/2145 nt)	yfcX ←	fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	2,462,869	1	.	T	89.4%	285.2 / 27.0	85	coding (1737/2145 nt)	yfcX	fused enoyl‑CoA hydratase, 3‑hydroxybutyryl‑CoA epimerase, dodecenoyl‑CoA D‑isomerase, and 3‑hydroxyacyl‑CoA dehydrogenase, subunit of anaerobic fatty acid oxidation complex
Reads supporting (aligned to +/- strand):  ref base . (9/0);  new base T (76/0);  total (85/0)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGATCGT  >  W3110S.gb/2462827‑2462891                                            |                       gCTTTTACGCCCTTTCTGACCATAAAGATAGAACCCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1306458/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:412145/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:580294/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:54460/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:494173/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:484132/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:455894/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:454625/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:452082/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:450413/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:435107/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:671736/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:402819/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:345772/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:340076/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:32913/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:29573/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:274071/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:248560/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:247548/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:230040/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:861234/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:995858/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:950568/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:948990/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:935324/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:901342/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:901296/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:882358/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:876774/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:867729/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:664783/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:822958/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:763810/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:761123/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:736096/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:719033/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:709751/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:694520/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:692367/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:215413/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1090391/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1560425/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1035982/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1527864/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1506497/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1081365/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1420385/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1377294/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1338868/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1571517/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1295394/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1123383/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1268891/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1246637/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1163915/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1224485/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1209910/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1194406/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1706597/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1020095/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1855687/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1855228/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1787653/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1767746/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:173070/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1730372/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1696862/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1688683/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1674905/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1666070/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1034442/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1605931/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1583888/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCATTTTTTTCTGCCTTTGCGAtc    >  1:1100474/1‑64 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑ttttttt                 >  1:1676451/1‑49 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGAtcgt  >  1:1498339/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGAtcgt  >  1:96905/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGAtcgt  >  1:798025/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGAtcgt  >  1:1278879/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGAtcgt  >  1:1870513/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGAtcgt  >  1:518848/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGAtcgt  >  1:163839/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGAtcgt  >  1:1007777/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGAtcgt  >  1:1882744/1‑65 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGCCCA‑t                       >  1:719963/1‑44 (MQ=255) gCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCAGGCCATTTTTTTCTGCCTTTGCGAtcg   >  1:1323734/1‑65 (MQ=255)                                            |                       GCTTTTACGCCCTTTCTGACCATAAAGATAGAAACCCCGGCCA‑TTTTTTCTGCCTTTGCGATCGT  >  W3110S.gb/2462827‑2462891

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑