Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	W3110S.gb	317,560	+T	coding (232/237 nt)	ykgI ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	W3110S.gb	317,557	1	.	T	100.0%	36.2 / NA	11	*79K (TAA→AAA)	ykgI	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base T (11/0);  total (11/0)

TTTTATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTA‑TTTATACAAT  >  W3110S.gb/317504‑317567                                                       |           ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:1062638/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:1126638/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:1308980/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:1464846/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:1548574/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:1752997/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:182448/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:1864338/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:202782/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTATTTTATACAAt  >  1:369953/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCATTATTTTATACAAt  >  1:376278/1‑65 (MQ=255) ttttATCTCCCCGACTTAACAGGTGCAGGTAGTTTTCCATTGTGAACATCCtta‑tttt        >  1:1530783/1‑57 (MQ=255)                                                       |           TTTTATCTCCCCGACTTAACAGGTGCAGGTATTTTTCCATTGTGAACATCCTTA‑TTTATACAAT  >  W3110S.gb/317504‑317567

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑